CHOP; GADD153 Antibody 2 Publications

Mouse Monoclonal| Catalog number: 60304-1-Ig |CloneNo.: 4D5A9

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Freight/Packing

Con: 210 μg/150 μl

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Species specificity:
human, mouse

Positive WB detected in:
Tunicamycin treated HeLa cells

Recommended dilution:
WB : 1:500-1:2000

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2a

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
DNA-damage-inducible transcript 3

Calculated molecular weight:
19 kDa

Observed molecular weight:
30 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
DDIT3

Synonyms
CEBPZ, CHOP, CHOP 10, CHOP10, DDIT 3, DDIT3, GADD153
Background

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions to hamster gene.Imposed by unfolded and malfolded proteins, CHOP is significantly induced by ER stress, deficiency of CHOP prevents cell from ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP by forming a heterodimer. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 30 kDa. Immunoprecipitation of FUS-DDIT3 from the myxoid liposarcoma cell lines using an anti-DDIT3 antibody. FUS-DDIT3 is detected in cell lines at around 75 kDa (PMID: 27270875). The calculated molecular weight of CHOP is 19 kDa, but aproteintha tmigrateson SDS-PAGE with an apparent molecular mass of 29 kDa (PMID: 1547942).


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