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CLCA2 Polyclonal antibody

CLCA2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19273-1-AP

Synonyms

CACC, CaCC 3, CACC3, chloride channel accessory 2, CLCA2, CLCRG2, hCaCC 3, hCLCA2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using CLCA2 Polyclonal antibody (19273-1-AP)

    WB analysis of mouse lung using 19273-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 19273-1-AP (CLCA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using CLCA2 Polyclonal antibody (19273-1-AP)

    WB analysis of COLO 320 using 19273-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19273-1-AP (CLCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using CLCA2 Polyclonal antibody (19273-1-AP)

    IHC staining of human oesophagus using 19273-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 19273-1-AP (CLCA2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using CLCA2 Polyclonal antibody (19273-1-AP)

    IHC staining of human oesophagus using 19273-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 19273-1-AP (CLCA2 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse lung tissue, COLO 320 cells
Positive IHC detected inhuman oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19273-1-AP is CLCA2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLCA2 fusion protein Ag5914
Full Name chloride channel accessory 2
Calculated Molecular Weight 104 kDa
Observed Molecular Weight 141 kDa, 35-40 kDa
GenBank Accession NumberBC041096
Gene Symbol CLCA2
Gene ID (NCBI) 9635
RRIDAB_10642000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CLCA2 antibody 19273-1-APDownload protocol
IHC protocol for CLCA2 antibody 19273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Front Cell Dev Biol

Epigenetic Repression of Chloride Channel Accessory 2 Transcription in Cardiac Fibroblast: Implication in Cardiac Fibrosis.

Authors - Tinghui Shao
View Article
mouseWB

Front Cardiovasc Med

Microenvironment Stiffness Amplifies Post-ischemia Heart Regeneration in Response to Exogenous Extracellular Matrix Proteins in Neonatal Mice.

Authors - Xinming Wang
View Article
humanIP

Biomedicines

Self-Cleavage of Human Chloride Channel Accessory 2 Causes a Conformational Shift That Depends on Membrane Anchorage and Is Required for Its Regulation of Store-Operated Calcium Entry

Authors - Grace T Ramena
View Article