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Claudin 7 Polyclonal antibody

Claudin 7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10118-1-AP

Synonyms

CEPTRL2, Claudin 7, CLDN 7, CLDN7, CPETRL2, Hs.84359

Validation Data Gallery

  • WB analysis of mouse liver using 10118-1-AP

    WB analysis of mouse liver using 10118-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10118-1-AP (Claudin 7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10118-1-AP

    IHC staining of human breast cancer using 10118-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10118-1-AP (Claudin 7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 10118-1-AP

    IHC staining of human breast cancer using 10118-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10118-1-AP (Claudin 7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

10118-1-AP targets Claudin 7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 7 fusion protein Ag0159
Full Name claudin 7
Calculated molecular weight22.4 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC001055
Gene symbol CLDN7
Gene ID (NCBI) 1366
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for Claudin 7 antibody 10118-1-APDownload protocol
IHC protocol for Claudin 7 antibody 10118-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Rep

The multi-targeted tyrosine kinase inhibitor vandetanib plays a bifunctional role in non-small cell lung cancer cells.

Authors - Yan Zhou
View Article