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Claudin 7 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 10118-1-AP

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Con: 37 μg/150 μl

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Tested Applications

Applications:
IHC, IF, ELISA
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Claudin 7 Antibody IHC human breast cancer tissue 10118-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10118-1-AP( Claudin 7 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Claudin 7 Antibody IHC human breast cancer tissue 10118-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10118-1-AP( Claudin 7 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Claudin 7 fusion protein Ag0159
Full name:
claudin 7
Calculated molecular weight:
23.5 kDa
Observed molecular weight:
GenBank accession number:
BC001055
Gene ID (NCBI):
1366
Gene symbol
CLDN7
Synonyms
CEPTRL2, Claudin 7, CLDN 7, CLDN7, CPETRL2, Hs.84359

Background

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.


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