Tested Applications
| Positive WB detected in | K-562 cells, Raji cells, mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84748-1-RR targets CLEC10A/CD301 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg1892 Product name: Recombinant Human CLEC10A/CD301 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 61-316 aa of BC039011 Sequence: QNSKFQRDLVTLRTDFSNFTSNTVAEIQALTSQGSSLEETIASLKAEVEGFKQERQAGVSELQEHTTQKAHLGHCPHCPSVCVPVHSEMLLRVQQLVQDLKKLTCQVATLNNNASTEGTCCPVNWVEHQDSCYWFSHSGMSWAEAEKYCQLKNAHLVVINSREEQNFVQKYLGSAYTWMGLSDPEGAWKWVDGTDYATGFQNWKPGQPDDWQGHGLGGGEDCAHFHPDGRWNDDVCQRPYHWVCEAGLGQTSQESH Predict reactive species |
| Full Name | C-type lectin domain family 10, member A |
| Calculated Molecular Weight | 316 aa, 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC039011 |
| Gene Symbol | CLEC10A |
| Gene ID (NCBI) | 10462 |
| RRID | AB_3672131 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q8IUN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
C-type lectin domain family 10 member A (CLEC10A), also known as CD301, macrophage galactose-type C-type lectin (MGL), DC-asialoglyco protein receptor (DC-ASGP-R) or human macrophage lectin (HML), is a type II transmembrane glycoprotein having a calcium-dependent carbohydrate recognition domain (PMID: 8598452; 11919201; 11698450). CLEC10A is expressed exclusively by myeloid professional antigen-presenting cells such as immature dendritic cells (DCs) and alternatively activated macrophages (PMID: 16998493). CLEC10A is a marker for human CD1c+ DCs (cDC2) (PMID: 29755453). It functions as an endocytic receptor for glycosylated antigens (PMID: 11919201).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CLEC10A/CD301 antibody 84748-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CLEC10A/CD301 Recombinant monoclonal antibody (241857C3), catalog number 84748-1-RR, has 1 total citation. It appears in Free Radical Biology and Medicine (IF: 8), published in 2025. The cited study investigates how 8-oxoguanine modifications in peripheral leukocyte microRNAs promote age-related inflammation by remodeling gene regulatory networks. The research field encompasses aging biology, oxidative stress, microRNA regulation, and inflammatory signaling. The antibody was applied in Western Blot (WB) using human samples.
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med 8-Oxoguanine Modifications in Peripheral Leukocyte microRNAs Promote Age-related Inflammation by Remodeling Gene Regulatory Networks |
