Tested Applications
| Positive WB detected in | mouse testis tissue, mouse lung tissue, mouse brain tissue, human brain tissue, human lung tissue, U-251 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12629-1-AP targets Calmegin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3322 Product name: Recombinant human CLGN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 311-610 aa of BC028357 Sequence: PAGWLDDEPKFIPDPNAEKPDDWNEDTDGEWEAPQILNPACRIGCGEWKPPMIDNPKYKGVWRPPLVDNPNYQGIWSPRKIPNPDYFEDDHPFLLTSFSALGLELWSMTSDIYFDNFIICSEKEVADHWAADGWRWKIMIANANKPGVLKQLMAAAEGHPWLWLIYLVTAGVPIALITSFCWPRKVKKKHKDTEYKKTDICIPQTKGVLEQEEKEEKAALEKPMDLEEEKKQNDGEMLEKEEESEPEEKSEEEIEIIEGQEESNQSNKSGSEDEMKEADESTGSGDGPIKSVRKRRVRKD Predict reactive species |
| Full Name | calmegin |
| Calculated Molecular Weight | 610 aa, 70 kDa |
| Observed Molecular Weight | 93 kDa |
| GenBank Accession Number | BC028357 |
| Gene Symbol | Calmegin |
| Gene ID (NCBI) | 1047 |
| RRID | AB_2276364 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14967 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Calmegin, belonging to the calreticulin family, is a testis-specific molecular chaperon of the endoplasmic reticulum playing a key role in spermatogenesis. Western blot analysis of mouse testis showed a single band with an apparent molecular mass of 93 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Calmegin antibody 12629-1-AP | Download protocol |
| IP protocol for Calmegin antibody 12629-1-AP | Download protocol |
| WB protocol for Calmegin antibody 12629-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Calmegin Polyclonal antibody (Cat# 12629-1-AP) has 3 total citations published in Molecular & Cellular Proteomics, Scientific Reports, and Journal of Biological Chemistry. The cited research focuses on mouse spermatogenesis and spermiogenesis, including post-transcriptional regulatory mechanisms of spermatogenesis, transcription factor RFX2 regulation of spermiogenesis, and ER-associated degradation adaptor SEL1L in spermatogenic cell differentiation.
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Integrative proteomic and transcriptomic analyses reveal multiple post-transcriptional regulatory mechanisms of mouse spermatogenesis. | ||
J Biol Chem The ER-associated Degradation Adaptor SEL1L is Dispensable for ER Homeostasis and the Differentiation of Spermatogenic Cells |
