Calmegin Polyclonal antibody

Calmegin Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12629-1-AP

Synonyms

calmegin, CLGN



Tested Applications

Positive WB detected inmouse testis tissue, human brain tissue, human lung tissue, mouse brain tissue, mouse lung tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12629-1-AP targets Calmegin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calmegin fusion protein Ag3322
Full Name calmegin
Calculated molecular weight 610 aa, 70 kDa
Observed molecular weight 93 kDa
GenBank accession numberBC028357
Gene symbol CLGN
Gene ID (NCBI) 1047
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calmegin, belonging to the calreticulin family, is a testis-specific molecular chaperon of the endoplasmic reticulum playing a key role in spermatogenesis. Western blot analysis of mouse testis showed a single band with an apparent molecular mass of 93 kDa.

Protocols

Product Specific Protocols
WB protocol for Calmegin antibody 12629-1-APDownload protocol
IHC protocol for Calmegin antibody 12629-1-APDownload protocol
IP protocol for Calmegin antibody 12629-1-APDownload protocol
FC protocol for Calmegin antibody 12629-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

Integrative proteomic and transcriptomic analyses reveal multiple post-transcriptional regulatory mechanisms of mouse spermatogenesis.

Authors - Haiyun Gan
mouseIF

Sci Rep

Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis.

Authors - Yujian Wu