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CLRN1 Polyclonal antibody

CLRN1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 26630-1-AP

Synonyms

clarin 1, CLRN1, USH3, USH3A, Usher syndrome type 3 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CLRN1 Polyclonal antibody (26630-1-AP)

    WB analysis of HeLa using 26630-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26630-1-AP (CLRN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CLRN1 Polyclonal antibody (26630-1-AP)

    IHC staining of mouse testis using 26630-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26630-1-AP (CLRN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CLRN1 Polyclonal antibody (26630-1-AP)

    IHC staining of mouse testis using 26630-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26630-1-AP (CLRN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CLRN1 Polyclonal antibody (26630-1-AP)

    IHC staining of mouse kidney using 26630-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26630-1-AP (CLRN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CLRN1 Polyclonal antibody (26630-1-AP)

    IHC staining of mouse kidney using 26630-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26630-1-AP (CLRN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse testis tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 26630-1-AP is CLRN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLRN1 fusion protein Ag24289
Full Name clarin 1
Calculated Molecular Weight 232 aa, 26 kDa
Observed Molecular Weight 23 kDa, 27 kDa
GenBank Accession NumberBC074970
Gene Symbol CLRN1
Gene ID (NCBI) 7401
RRIDAB_2880579
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CLRN1 antibody 26630-1-APDownload protocol
IHC protocol for CLRN1 antibody 26630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Pathol

Clarin-1 expression in adult mouse and human retina highlights a role of Müller glia in Usher syndrome.

Authors - Lei Xu
View Article