|Positive WB detected in||NIH/3T3 cells|
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10313-1-AP targets CNOT2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CNOT2 fusion protein Ag0347|
|Full Name||CCR4-NOT transcription complex, subunit 2|
|Calculated molecular weight||60 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC002597|
|Gene ID (NCBI)||4848|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The Ccr4–Not complex is evolutionarily conserved and controls gene expression both at the level of transcription initiation and of mRNA degradation. And this complex can regulate pol II transcription. Promoter targeting of CCR4-NOT transcription complex subunit 2 (CNOT2) results in strong repression of pol II transcription. CNOT2-mediated repression is sensitive to the HDAC (histone deacetylase) inhibitor, TSA (trichostatin A).
Stem Cells Dev
Identification of Ccr4-Not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.
Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability.
Stem Cell Reports
CNOT3-Dependent mRNA Deadenylation Safeguards the Pluripotent State.
Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation.