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  • KD/KO Validated

CNOT2 Polyclonal antibody

CNOT2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 10313-1-AP

Synonyms

CCR4 associated factor 2, CDC36, CNOT2, HSPC131, MSTP046, NOT2, NOT2H



Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10313-1-AP targets CNOT2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT2 fusion protein Ag0347
Full Name CCR4-NOT transcription complex, subunit 2
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC002597
Gene symbol CNOT2
Gene ID (NCBI) 4848
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Ccr4–Not complex is evolutionarily conserved and controls gene expression both at the level of transcription initiation and of mRNA degradation. And this complex can regulate pol II transcription. Promoter targeting of CCR4-NOT transcription complex subunit 2 (CNOT2) results in strong repression of pol II transcription. CNOT2-mediated repression is sensitive to the HDAC (histone deacetylase) inhibitor, TSA (trichostatin A).

Protocols

Product Specific Protocols
WB protocol for CNOT2 antibody 10313-1-APDownload protocol
IF protocol for CNOT2 antibody 10313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Stem Cells Dev

Identification of Ccr4-Not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.

Authors - Kamon Masayoshi M
  • KD Validated
mouseWB

RNA Biol

Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability.

Authors - Dina Mostafa
mouseWB

Stem Cell Reports

CNOT3-Dependent mRNA Deadenylation Safeguards the Pluripotent State.

Authors - Xiaofeng Zheng
human,mouseWB

Stem Cells

Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation.

Authors - Zheng Xiaofeng X
  • KD Validated