Collagen Type VI Polyclonal antibody

Collagen Type VI Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17023-1-AP

Synonyms

COL6A1, collagen 6A1, Collagen alpha 1(VI) chain, Collagen Type VI, collagen, type VI, alpha 1, OPLL



Tested Applications

Positive WB detected inmouse heart tissue, human skeletal muscle tissue, human heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue, mouse liver tissue, human lung tissue, human ovary tissue, human hepatocirrhosis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17023-1-AP targets Collagen Type VI in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type VI fusion protein Ag10288
Full Name collagen, type VI, alpha 1
Calculated molecular weight 1028 aa, 109 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC052575
Gene symbol COL6A1
Gene ID (NCBI) 1291
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

Protocols

Product Specific Protocols
WB protocol for Collagen Type VI antibody 17023-1-APDownload protocol
IHC protocol for Collagen Type VI antibody 17023-1-APDownload protocol
IP protocol for Collagen Type VI antibody 17023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Physiol

Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.

Authors - He Wang
mouseWB

Exp Cell Res

Proteomic analysis of mouse ovaries during the prepubertal stages.

Authors - Jiaqiang Xiong

Methods Mol Biol

Gapmer Antisense Oligonucleotides to Selectively Suppress the Mutant Allele in COL6A Genes in Dominant Ullrich Congenital Muscular Dystrophy.

Authors - Sara Aguti
humanWB,IF

BMC Res Notes

Authentication of collagen VI antibodies.

Authors - Jamie Endicott
WB

Eur J Dermatol

Differential effect of extracellular matrix derived from papillary and reticular fibroblasts on epidermal development in vitro.

Authors - David Janson

PLoS One

A new approach for the study of lung smooth muscle phenotypes and its application in a murine model of allergic airway inflammation.

Authors - Paez-Cortez Jesus J

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Malak (Verified Customer) (02-24-2021)

It works well in the western blot

  • Applications: Western Blot