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  • KD/KO Validated

COMMD1 Polyclonal antibody

COMMD1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 11938-1-AP

Synonyms

C2orf5, COMMD1, MURR1, Protein Murr1



Tested Applications

Positive WB detected inhuman liver tissue, HeLa cells, HepG2 cells, human brain tissue, human heart tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11938-1-AP targets COMMD1 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity canine, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COMMD1 fusion protein Ag2535
Full Name copper metabolism (Murr1) domain containing 1
Calculated molecular weight 190 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC022046
Gene symbol COMMD1
Gene ID (NCBI) 150684
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.

Protocols

Product Specific Protocols
WB protocol for COMMD1 antibody 11938-1-APDownload protocol
IHC protocol for COMMD1 antibody 11938-1-APDownload protocol
IF protocol for COMMD1 antibody 11938-1-APDownload protocol
IP protocol for COMMD1 antibody 11938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Biochim Biophys Acta

A cell-type-specific role for murine Commd1 in liver inflammation.

Authors - Paulina Bartuzi
mouseWB,IHC

Biol Trace Elem Res

The Alteration of Copper Homeostasis in Inflammation Induced by Lipopolysaccharides.

Authors - Han Ming M
humanWB,IHC

PLoS One

PMID: 27788210

Authors - Alina Fedoseienko
  • KD Validated
humanWB,IF

PLoS One

COMMD1-mediated ubiquitination regulates CFTR trafficking.

Authors - Drévillon Loïc L
humanWB

Int J Biochem Cell Biol

COMMD1 modulates noxious inflammation in cystic fibrosis.

Authors - de Becdelièvre Alix A
mouseWB

Biochem J

Roles of COMM-domain-containing 1 in stability and recruitment of the copper-transporting ATPase in a mouse hepatoma cell line.

Authors - Miyayama Takamitsu T
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emilia (Verified Customer) (11-30-2018)

works well for WB

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1;1000
  • Cell Tissue Type: 293, Cos1