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WB analysis using 11457-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 11457-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human stomach cancer using 11457-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 11457-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 11457-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 11457-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11457-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate cancer using 11457-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 11457-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 11457-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 11457-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COPE antibody (11457-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

COPE Polyclonal antibody

COPE Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11457-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Coatomer subunit epsilon, Epsilon-coat protein, Epsilon-COP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, A375 cells, HEK-293 cells, Jurkat cells, MCF-7 cells, PC-3 cells
Positive IHC detected in	human stomach cancer tissue, human liver cancer tissue, human prostate cancer tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11457-1-AP targets COPE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2024 Product name: Recombinant human COPE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-308 aa of BC017285 Sequence: MAPPAPGPASGGSGEVDELFDVKNAFYIGSYQQCINEAQRVKLSSPERDVERDVFLYRAYLAQRKFGVVLDEIKPSSAPELQAVRMFADYLAHESRRDSIVAELDREMSRSVDVTNTTFLLMAASIYLHDQNPDAALRALHQGDSLECTAMTVQILLKLDRLDLARKELKRMQDLDEDATLTQLATAWVSLATGGEKLQDAYYIFQEMADKCSPTLLLLNGQAACHMAQGRWEAAEGLLQEALDKDSGYPETLVNLIVLSQHLGKPPEVTNRYLSQLKDAHRSHPFIKEYQAKENDFDRLVLQYAPSA Predict reactive species
Full Name	coatomer protein complex, subunit epsilon
Calculated Molecular Weight	34 kDa
Observed Molecular Weight	30-35 kDa
GenBank Accession Number	BC017285
Gene Symbol	COPE
Gene ID (NCBI)	11316
RRID	AB_11182386
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14579
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for COPE antibody 11457-1-AP	Download protocol
WB protocol for COPE antibody 11457-1-AP	Download protocol
IF protocol for COPE antibody 11457-1-AP	Download protocol