COPE Polyclonal antibody

COPE Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11457-1-AP

Synonyms

Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP



Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells, A375 cells, HeLa cells, A431 cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11457-1-AP targets COPE in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPE fusion protein Ag2024
Full Name coatomer protein complex, subunit epsilon
Calculated molecular weight 34 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC017285
Gene symbol COPE
Gene ID (NCBI) 11316
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COPE antibody 11457-1-APDownload protocol
IHC protocol for COPE antibody 11457-1-APDownload protocol
IF protocol for COPE antibody 11457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols