• Featured Product
  • KD/KO Validated

COQ4 Polyclonal antibody

COQ4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16654-1-AP

Synonyms

CGI 92, COQ4



Tested Applications

Positive WB detected inmouse liver tissue, Transfected HEK-293 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16654-1-AP targets COQ4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COQ4 fusion protein Ag10089
Full Name coenzyme Q4 homolog (S. cerevisiae)
Calculated molecular weight 265 aa, 30 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC011895
Gene symbol COQ4
Gene ID (NCBI) 51117
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for COQ4 antibody 16654-1-APDownload protocol
IHC protocol for COQ4 antibody 16654-1-APDownload protocol
IP protocol for COQ4 antibody 16654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO Mol Med

β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.

Authors - Agustín Hidalgo-Gutiérrez
mouseWB

Redox Biol

The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency.

Authors - Pilar González-García
humanWB

J Med Genet

Human COQ4 deficiency: delineating the clinical, metabolic and neuroimaging phenotypes.

Authors - Lucia Laugwitz
humanWB

Biochim Biophys Acta Bioenerg

Characterization of human mitochondrial PDSS and COQ proteins and their roles in maintaining coenzyme Q10 levels and each other's stability.

Authors - Hsiu-Chuan Yen
humanWB

Mol Genet Metab

Detection of 6-demethoxyubiquinone in CoQ10 deficiency disorders: Insights into enzyme interactions and identification of potential therapeutics.

Authors - Diran Herebian
  • KD Validated
humanWB

Ann Clin Transl Neurol

4-Hydroxybenzoic acid restores CoQ10 biosynthesis in human COQ2 deficiency.

Authors - Diran Herebian