|Positive WB detected in||A549 cells, mouse lung tissue|
|Positive IHC detected in||human lung tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse lung tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 27308-1-AP is COX2/ Cyclooxygenase 2/ PTGS2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag26174|
|Full Name||prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)|
|Calculated molecular weight||604 aa, 68 kDa|
|Observed molecular weight||69 kDa|
|GenBank accession number||BC013734|
|Gene ID (NCBI)||5743|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa(PMID:19656660 ). It also expresses a band of 39 kDa after unspecific cleavage(PMID:17509125 ). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains(PMID:14724276 ).
|Product Specific Protocols|
|WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-AP||Download protocol|
|IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-AP||Download protocol|
|IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ryan (Verified Customer) (02-27-2019)
Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown).