Tested Applications
| Positive WB detected in | HeLa cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 13 publications below |
| IF | See 4 publications below |
Product Information
11418-2-AP targets COX5B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1986 Product name: Recombinant human COX5B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-129 aa of BC006229 Sequence: MASRLLRGAGTLAAQALRARGPSGAAAMRSMASGGGVPTDEEQATGLEREIMLAAKKGLDPYNVLAPKGASGTREDPNLVPSISNKRIVGCICEEDNTSVVWFWLHKGEAQRCPRCGAHYKLVPQQLAH Predict reactive species |
| Full Name | cytochrome c oxidase subunit Vb |
| Calculated Molecular Weight | 129 aa, 14 kDa |
| Observed Molecular Weight | 14 kDa |
| GenBank Accession Number | BC006229 |
| Gene Symbol | COX5B |
| Gene ID (NCBI) | 1329 |
| RRID | AB_2245376 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10606 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COX5B antibody 11418-2-AP | Download protocol |
| IHC protocol for COX5B antibody 11418-2-AP | Download protocol |
| WB protocol for COX5B antibody 11418-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.
| ||
Nat Immunol Mitochondrial respiratory-chain adaptations in macrophages contribute to antibacterial host defense. | ||
Cell Metab Enhanced Respiratory Chain Supercomplex Formation in Response to Exercise in Human Skeletal Muscle. | ||
Mol Cell Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Süleyman (Verified Customer) (02-07-2022) | HeLa cell line is used and even though it has some unspecific bands but it is very specific and the unspecific bands are very distant. Recommended!
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