COXIV Monoclonal antibody

COXIV Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3C7D2

Cat no : 60251-1-Ig

Synonyms

Cell and organelle markers, COX IV 1, COX4, COX4I1, COXIV, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60251-1-Ig is COXIV Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COXIV fusion protein Ag20551
Full Name cytochrome c oxidase subunit IV isoform 1
Calculated molecular weight19.6 kDa
Observed molecular weight 17-18 kDa
GenBank accession numberBC021236
Gene symbol COX4I1
Gene ID (NCBI) 1327
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.

Protocols

Product Specific Protocols
IHC protocol for COXIV antibody 60251-1-IgDownload protocol
IF protocol for COXIV antibody 60251-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Hypertension

Upregulation of Piezo1 (Piezo Type Mechanosensitive Ion Channel Component 1) Enhances the Intracellular Free Calcium in Pulmonary Arterial Smooth Muscle Cells From Idiopathic Pulmonary Arterial Hypertension Patients.

Authors - Jing Liao
mouseIHC,IF

Mol Metab

Disruption of STIM1-mediated Ca2+ sensing and energy metabolism in adult skeletal muscle compromises exercise tolerance, proteostasis and lean mass.

Authors - Rebecca J Wilson
mouseIF

Mol Cell Biol

Protein Kinase A/CREB Signaling Prevents Adriamycin-Induced Podocyte Apoptosis via Upregulation of Mitochondrial Respiratory Chain Complexes.

Authors - Kewei Xie
mouseWB,IF

Biochem Biophys Res Commun

Dopamine receptor D1 signaling stimulates lipolysis and browning of white adipocytes.

Authors - Jing Yu
ratWB,IF

Eur J Histochem

Transcription factor nuclear factor erythroid 2 p45-related factor 2 (NRF2) ameliorates sepsis-associated acute kidney injury by maintaining mitochondrial homeostasis and improving the mitochondrial function.

Authors - Zhijiang Chen
ratWB

Cells

Parkin-Dependent Mitophagy is Required for the Inhibition of ATF4 on NLRP3 Inflammasome Activation in Cerebral Ischemia-Reperfusion Injury in Rats.

Authors - Qi He