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COXIV Antibody 2 Publications

Mouse Monoclonal| Catalog number: 60251-1-Ig |CloneNo.: 3C7D2

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.


Images See all

COXIV Antibody WB HEK-293 cells 60251-1-Ig

HEK-293 cells were subjected to SDS PAGE followed by western blot with 60251-1-Ig(COXIV antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

COXIV Antibody IHC human prostate cancer tissue 60251-1-Ig

Immunohistochemical analysis of paraffin-embedded human prostate cancer using 60251-1-Ig(COXIV antibody) at dilution of 1:100 (under 10x lens)

COXIV Antibody IHC human prostate cancer tissue 60251-1-Ig

Immunohistochemical analysis of paraffin-embedded human prostate cancer using 60251-1-Ig(COXIV antibody) at dilution of 1:100 (under 40x lens)

COXIV Antibody IF HepG2 cells 60251-1-Ig

Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 60251-1-Ig (COXIV antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

COXIV Antibody FC HeLa cells 60251-1-Ig

1X10^6 HeLa cells were stained with 0.2ug COXIV antibody (60251-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells
Positive IHC detected in:
human prostate cancer tissue, human heart tissue, human pancreas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
COXIV fusion protein Ag20551
Full name:
cytochrome c oxidase subunit IV isoform 1
Calculated molecular weight:
19.6 kDa
Observed molecular weight:
17-18 kDa
GenBank accession number:
BC021236
Gene ID (NCBI):
1327
Gene symbol
COX4I1
Synonyms
COX IV 1, COX4, COX4I1, COXIV, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial

Background

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.


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