Alpha N-Catenin Polyclonal antibody
Alpha N-Catenin Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14362-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | SH-SY5Y cells, mouse pancreas tissue |
Positive IP detected in | SH-SY5Y cells |
Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
14362-1-AP targets Alpha N-Catenin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Alpha N-Catenin fusion protein Ag5747 |
Full Name | catenin (cadherin-associated protein), alpha 2 |
Calculated Molecular Weight | 105 kDa |
Observed Molecular Weight | 102 kDa |
GenBank Accession Number | BC052996 |
Gene Symbol | CTNNA2 |
Gene ID (NCBI) | 1496 |
RRID | AB_2087961 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for Alpha N-Catenin antibody 14362-1-AP | Download protocol |
IHC protocol for Alpha N-Catenin antibody 14362-1-AP | Download protocol |
IF protocol for Alpha N-Catenin antibody 14362-1-AP | Download protocol |
IP protocol for Alpha N-Catenin antibody 14362-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |