Alpha N-Catenin Polyclonal antibody

Alpha N-Catenin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14362-1-AP

Synonyms

alpha 2 Catenin, Alpha catenin related protein, Alpha N catenin, Alpha N-Catenin, Catenin alpha 2, CTNNA2, CTNR



Tested Applications

Positive WB detected inSH-SY5Y cells, mouse pancreas tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14362-1-AP targets Alpha N-Catenin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha N-Catenin fusion protein Ag5747
Full Name catenin (cadherin-associated protein), alpha 2
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC052996
Gene Symbol CTNNA2
Gene ID (NCBI) 1496
RRIDAB_2087961
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Alpha N-Catenin antibody 14362-1-APDownload protocol
IHC protocol for Alpha N-Catenin antibody 14362-1-APDownload protocol
IF protocol for Alpha N-Catenin antibody 14362-1-APDownload protocol
IP protocol for Alpha N-Catenin antibody 14362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols