CXCR1 Polyclonal antibody

CXCR1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 55450-1-AP

Synonyms

C C, C C CKR 1, CD128, CD181, CDw128a, CKR 1, CMKAR1, CXC R1, CXCR 1, CXCR1, IL 8 receptor type 1, IL 8R A, IL8R1, IL8RA, IL-8RA, IL8RBA, interleukin 8 receptor, alpha



Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55450-1-AP targets CXCR1 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name interleukin 8 receptor, alpha
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberNM_000634
Gene Symbol IL8RA
Gene ID (NCBI) 3577
RRIDAB_11182706
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCR1, also named as CMKAR1, IL8RA and CD181, belongs to the G-protein coupled receptor 1 family. It is a receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. CXCR1binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity. This antibody is specific to CXCR1.

Protocols

Product Specific Protocols
WB protocol for CXCR1 antibody 55450-1-APDownload protocol
IP protocol for CXCR1 antibody 55450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Dis

CXCR1 drives the pathogenesis of EAE and ARDS via boosting dendritic cells-dependent inflammation

Authors - Wei Zhuang
mouseWB,IF

Cell Rep

Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism

Authors - Hao Qin
  • KD Validated
humanWB

Lung Cancer

Combination therapy with anti-programmed cell death 1 antibody plus angiokinase inhibitor exerts synergistic antitumor effect against malignant mesothelioma via tumor microenvironment modulation

Authors - Akio Tada
mouseWB

Cytokine

IL-8 exacerbates alcohol-induced fatty liver disease via the Akt/HIF-1α pathway in human IL-8-expressing mice.

Authors - Zhihao Wang
mouseWB

J Agric Food Chem

Oral Intervention of Narirutin Ameliorates the Allergic Response of Ovalbumin Allergy

Authors - Xiaolei Shi
mouseWB

Mol Immunol

IL-8 exacerbates CCl4-induced liver fibrosis in human IL-8-expressing mice via the PI3K/Akt/HIF-1α pathway

Authors - Yuchen Ma