CXCR3 Antibody

CXCR3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 26756-1-AP

Synonyms

CD183, CKR L2, CMKAR3, CXC R3, CXCR 3, CXCR3, CXCR3A, CXCR3B, G protein coupled receptor 9, GPR9, IP 10 receptor, IP10 R, Mig R, MigR



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, mouse liver tissue
Positive IHC detected inhuman liver tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 26756-1-AP immunogen is Fusion Protein CXCR3 protein expressed in E. coli.

Reactivity human, mouse
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCR3 fusion protein Ag25056
Full Name chemokine (C-X-C motif) receptor 3
Calculated molecular weight 368aa,41 kDa
Observed molecular weight 70 kDa
GenBank accession number BC034403
Gene symbol CXCR3
Gene ID (NCBI) 2833
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

CXCR3 (C-X-C chemokine receptor type 3) is a G-protein-coupled seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells. CXCR3 is activated by three related chemokines (CXCL9, CXCL10, and CXCL11) and plays an important role in effector T-cell and NK cell trafficking. CXCR3 has three isoforms termed as CXCR3-A, CXCR3-B and CXCR3-alt with the calculated molecular mass of 41kDa, 46kDa and 29kDa respectively. The apparent molecular mass of CXCR3 detected by some scientists is 70kDa, but 45kDa by some others. (PMID: 16847335, 15150261, 22885102, 19151743)

Protocols

Product Specific Protocols
WB protocol for CXCR3 antibody 26756-1-APDownload protocol
IHC protocol for CXCR3 antibody 26756-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Br J Pharmacol

Phosphodiesterase-4 inhibition attenuates murine ulcerative colitis via interfering with mucosal immunity.

Authors - Heng Li
humanWB

Autoimmunity

CXCL4 promoted the production of CD4+CD25+FOXP3+treg cells in mouse sepsis model through regulating STAT5/FOXP3 pathway.

Authors - Tao Xu
humanWB

J Cell Sci

Gap junction-mediated MiR-200b on osteogenesis and angiogenesis in coculture between MSCs and HUVECs.

Authors - Xiaoting Fan