CXCR4 Polyclonal antibody

CXCR4 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11073-2-AP

Synonyms

CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM



Tested Applications

Positive WB detected inrat spleen tissue, Jurkat cells, HeLa cells, mouse spleen tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

11073-2-AP targets CXCR4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCR4 fusion protein Ag1528
Full Name chemokine (C-X-C motif) receptor 4
Calculated molecular weight 352 aa, 40 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC020968
Gene symbol CXCR4
Gene ID (NCBI) 7852
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some researches, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).

Protocols

Product Specific Protocols
WB protocol for CXCR4 antibody 11073-2-APDownload protocol
IP protocol for CXCR4 antibody 11073-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

CNS Neurosci Ther

CXCL12/CXCR4 signaling contributes to neuropathic pain via central sensitization mechanisms in a rat spinal nerve ligation model.

Authors - Zhi-Yuan Liu
humanWB

J Exp Clin Cancer Res

Pre-metastatic niche triggers SDF-1/CXCR4 axis and promotes organ colonisation by hepatocellular circulating tumour cells via downregulation of Prrx1.

Authors - Yujun Tang
humanWB

Tumour Biol

The SDF-1/CXCR4 chemokine axis in uveal melanoma cell proliferation and migration.

Authors - Jianjun Bi
humanWB

Cell Physiol Biochem

The Natural Compound Myricetin Effectively Represses the Malignant Progression of Prostate Cancer by Inhibiting PIM1 and Disrupting the PIM1/CXCR4 Interaction.

Authors - Chen Ye
mouseWB

Evid Based Complement Alternat Med

Electroacupuncture at ST36 Increases Bone Marrow-Derived Interstitial Cells of Cajal via the SDF-1/CXCR4 and mSCF/Kit-ETV1 Pathways in the Stomach of Diabetic Mice.

Authors - Jiao Zhao
humanWB,IHC

Am J Transl Res

Suppression of chronic lymphocytic leukemia progression by CXCR4 inhibitor WZ811.

Authors - Shi Hui Li