CYP3A4 Antibody

CYP3A4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18227-1-AP

Synonyms

Albendazole monooxygenase, Albendazole sulfoxidase, CP33, CP34, CYP3A, CYP3A11, CYP3A3, CYP3A4, CYPIIIA3, CYPIIIA4, Cytochrome P450 3A3, Cytochrome P450 3A4, Cytochrome P450 HLp, Cytochrome P450 NF 25, Cytochrome P450 PCN1, HLP, NF 25, Nifedipine oxidase, P450C3, P450PCN1, Quinine 3 monooxygenase



Tested Applications

Positive WB detected inrat liver tissue, mouse liver tissue, mouse brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18227-1-AP targets CYP3A4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP3A4 fusion protein Ag13074
Full Name cytochrome P450, family 3, subfamily A, polypeptide 4
Calculated molecular weight 503aa,57 kDa
Observed molecular weight 51-57 kDa
GenBank accession number BC101631
Gene symbol CYP3A4
Gene ID (NCBI) 1576
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytochrome P450 3A(CYP3A) belongs to the cytochrome P450 family and the enzymes constitute an important detoxification system that contributes to primary metabolism of more than half of all prescribed medications.CYP3A expression determines impairment of drug absorption and efficient systemic clearance in a tissue-specific manner(PMID:17975676). The murine homolog of human CYP3A4 is CYP3A11 (PMID: 19297428). The highest amounts of drug-metabolizing cytochromes P-450 are found in the liver and intestine, with lower amounts found in other organs, including the kidney, lung, and brain. LPS that has reached the circulation stimulates a TLR4-dependent mechanism in the periphery, evoking a reduction in Cyp3a11 expression and metabolism in the liver (PMID: 15878984).

Protocols

Product Specific Protocols
WB protocol for CYP3A4 antibody 18227-1-APDownload protocol
IHC protocol for CYP3A4 antibody 18227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Regen Ther

Novel hybrid three-dimensional artificial liver using human induced pluripotent stem cells and a rat decellularized liver scaffold.

Authors - Takahito Minami
mouseWB

Toxicol Sci

Editor's Highlight: Pregnancy Alters Aflatoxin B1 Metabolism and Increases DNA Damage in Mouse Liver.

Authors - Kanokwan Sriwattanapong

Oncotarget

Alpha-ketoglutarate suppresses the NF-κB-mediated inflammatory pathway and enhances the PXR-regulated detoxification pathway.

Authors - Liuqin He
mouse

Xenobiotica

Effects of tetrahydroxystilbene glucoside on mouse liver cytochrome P450 enzyme expressions.

Authors - Yan-Ying Wang
mouseWB

Biopharm Drug Dispos

Characterization of red ginseng-drug interaction by CYP3A activity increased in high dose administration in mice.

Authors - Youn Ah Kim
humanWB

J Virol Methods

Long-term maintenance of human fetal hepatocytes and prolonged susceptibility to HBV infection by co-culture with non-parenchymal cells.

Authors - Zhou Ming M