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Connexin 43 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 26980-1-AP

1 customer review

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

Connexin 43 Antibody WB mouse testis tissue 26980-1-AP

mouse testis tissue were subjected to SDS PAGE followed by western blot with 26980-1-AP (Connexin 43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Connexin 43 Antibody IHC mouse heart tissue 26980-1-AP

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26980-1-AP( Connexin 43 Antibody) at dilution of 1:200 (under 10x lens).

Connexin 43 Antibody IHC mouse heart tissue 26980-1-AP

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26980-1-AP( Connexin 43 Antibody) at dilution of 1:200 (under 40x lens).

Connexin 43 Antibody IF mouse heart tissue 26980-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse heart tissue using 26980-1-AP (Connexin 43 antibody) at dilution of 1:50 and 66895-1-Ig (alpha Actinin mouse mAb) at dilution of 1:50, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 26980-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66895-1-Ig.

Connexin 43 Antibody IP mouse heart tissue 26980-1-AP

IP result of anti-Connexin 43(IP:26980-1-AP, 4ug; Detection:26980-1-AP 1:500) with mouse heart tissue lysate 4000 ug

Species specificity:
human, mouse , rat
Positive WB detected in:
mouse testis tissue
Positive IP detected in:
mouse heart tissue
Positive IHC detected in:
mouse heart tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse heart tissue, human heart tissue
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Connexin 43 fusion protein Ag25533
Full name:
gap junction protein, alpha 1, 43kDa
Calculated molecular weight:
43 kDa
Observed molecular weight:
43-47 kDa
GenBank accession number:
BC026329
Gene ID (NCBI):
2697
Gene symbol
GJA1
Synonyms
Connexin 43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD

Background

Connexin-43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

Connexin 43 Rabbit Polyclonal antibody

ABDULLAH (Verified Customer), 11-08-2019
Applications
Western Blot,Immunofluorescence,
Primary Antibody Dilution
1:1000
No image submitted with review

No image submitted with review

Cell/Tissue Type
BEND.3 cells
Lot Number
00049951
This antibody worked great! It detected the protein at the right size. There wasn't any background.

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