mouse skin tissue were subjected to SDS PAGE followed by western blot with 86325-2-RR (KRT74 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
mouse skin tissue were subjected to SDS PAGE followed by western blot with 86325-2-RR (KRT74 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Cytokeratin 74 (KRT74) is a type II keratin protein, specifically found in the inner root sheath of hair follicles. It's crucial for hair formation and maintaining the structural integrity of hair. Mutations in the KRT74 gene can lead to various hair disorders, including woolly hair and hypotrichosis.
Protocols
Product Specific Protocols
WB protocol for Cytokeratin 74 antibody 86325-2-RR
mouse skin tissue were subjected to SDS PAGE followed by western blot with 86325-2-RR (KRT74 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Cytokeratin 74 Recombinant antibody
Catalog Number
86325-2-RR
Citations
-
Dilutions
WB : 1:1000-1:4000
Applications
WB, ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet