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DAND5 Polyclonal antibody

DAND5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 22137-1-AP

Synonyms

CER2, Cerberus like protein 2, Cerl 2, CERL2, CKTSF1B3, COCO, CRL2, DAN domain family member 5, DAN domain family, member 5, DAND5, DANTE, GREM3, Gremlin 3, SP1

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using DAND5 Polyclonal antibody (22137-1-AP)

    WB analysis of human heart using 22137-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 22137-1-AP (DAND5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using DAND5 Polyclonal antibody (22137-1-AP)

    IHC staining of human testis using 22137-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using DAND5 Polyclonal antibody (22137-1-AP)

    IHC staining of human testis using 22137-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human heart tissue using DAND5 Polyclonal antibody (22137-1-AP)

    IHC staining of human heart using 22137-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human heart tissue using DAND5 Polyclonal antibody (22137-1-AP)

    IHC staining of human heart using 22137-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22137-1-AP is DAND5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAND5 fusion protein Ag17533
Full Name DAN domain family, member 5
Calculated Molecular Weight 189 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC113476
Gene Symbol DAND5
Gene ID (NCBI) 199699
RRIDAB_2879004
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DAND5 antibody 22137-1-APDownload protocol
IHC protocol for DAND5 antibody 22137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols