DARS Polyclonal antibody

DARS Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14989-1-AP

Synonyms

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14989-1-AP targets DARS in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DARS fusion protein Ag6974
Full Name aspartyl-tRNA synthetase
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 50-57 kDa
GenBank Accession NumberBC000629
Gene Symbol DARS
Gene ID (NCBI) 1615
RRIDAB_2261349
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DARS antibody 14989-1-APDownload protocol
IHC protocol for DARS antibody 14989-1-APDownload protocol
IP protocol for DARS antibody 14989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseCoIP

J Virol

Multiple Host Factors Interact with Hypervariable Domain of Chikungunya Virus nsP3 and Determine Viral Replication in Cell-Specific Mode.

Authors - Chetan D Meshram
humanWB

Am J Cancer Res

hsa_circ_0000519 promotes the progression of lung adenocarcinoma through the hsa-miR-1296-5p/DARS axis

Authors - Guangxu Tu