Tested Applications
| Positive WB detected in | SH-SY5Y cells, NIH/3T3 cells, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | rat brain tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10777-1-AP targets Dopamine beta Hydroxylase in WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1196 Product name: Recombinant human DBH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 269-603 aa of BC017174 Sequence: CDSKMKPDRLNYCRHVLAAWALGAKAFYYPEEAGLAFGGPGSSRYLRLEVHYHNPLVIEGRNDSSGIRLYYTAKLRRFNAGIMELGLVYTPVMAIPPRETAFILTGYCTDKCTQLALPPSGIHIFASQLHTHLTGRKVVTVLVRDGREWEIVNQDNHYSPHFQEIRMLKKVVSVHPGDVLITSCTYNTEDRELATVGGFGILEEMCVNYVHYYPQTQLELCKSAVDAGFLQKYFHLINRFNNEDVCTCPQASVSQQFTSVPWNSFNRDVLKALYSFAPISMHCNKSSAVRFQGEWNLQPLPKVISTLEEPTPQCPTSQGRSPAGPTVVSIGGGKG Predict reactive species |
| Full Name | DBH |
| Calculated Molecular Weight | 64.8 kDa |
| Observed Molecular Weight | 63 kDa |
| GenBank Accession Number | BC017174 |
| Gene Symbol | DBH |
| Gene ID (NCBI) | 1621 |
| RRID | AB_2277037 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09172 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DBH belongs to the copper type II ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of DBH to norepinephrine. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons(PMID:11857564).The DBH is glycosylated (63--67 kDa) while still in the membrane(PMID:2325165) and it can form a dimer(PMID:8546710).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Dopamine beta Hydroxylase antibody 10777-1-AP | Download protocol |
| IF protocol for Dopamine beta Hydroxylase antibody 10777-1-AP | Download protocol |
| IHC protocol for Dopamine beta Hydroxylase antibody 10777-1-AP | Download protocol |
| WB protocol for Dopamine beta Hydroxylase antibody 10777-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Dopamine beta Hydroxylase (DBH) Polyclonal antibody (Cat# 10777-1-AP) has 6 total citations published in Nature Chemical Biology, Neurobiology of Disease, International Journal of Molecular Sciences, Peptides, Tissue Cell, and PLoS One. The research fields span neurochemistry and norepinephrine imaging, neurodevelopmental defects and ferroptosis in zebrafish, neuroblastoma biology, diabetic choroid pathology, autonomic nervous system imbalance, and noradrenergic markers in cancer-induced bone pain.
| Species | Application | Title |
|---|---|---|
Nat Chem Biol Designing chemigenetic DNA nanotrap for norepinephrine dynamic imaging in organelles. | ||
Neurobiol Dis Atp7a deficiency induces axonal and myelin developmental defects in zebrafish via ferroptosis. | ||
Int J Mol Sci Intermittent Hypoxia Increased the Expression of DBH and PNMT in Neuroblastoma Cells via MicroRNA-375-Mediated Mechanism. | ||
Peptides Markers of the sympathetic, parasympathetic and sensory nervous system are altered in the human diabetic choroid. | ||
PLoS One Alteration of BDNF and noradrenergic markers in locus coeruleus in a mouse model of cancer-induced bone pain. |
Reviews
What customers say
One customer review is available for this product. Mounika V. gave it a 5-star rating in December 2025, reporting successful use in Western Blot, Immunohistochemistry, and ChIP applications with a dilution of 1:600 (lot 00018432). No additional written comments were provided, but the top rating across multiple validated applications suggests strong performance and reliability for this antibody.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (12-25-2025) |
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