Various lysates were subjected to SDS PAGE followed by western blot with 10777-1-AP (DBH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 10777-1-AP (DBH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 10777-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 10777-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10777-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 10x lens).
IHC staining of human liver cancer using 10777-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 40x lens).
IF Staining of rat brain using 10777-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using DBH antibody (10777-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using DBH antibody (10777-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of SH-SY5Y using 10777-1-AP
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DBH antibody (10777-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DBH antibody (10777-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HeLa using 10777-1-AP
Immunofluorescent analysis of HeLa cells using 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of HeLa cells using 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of SH-SY5Y using 10777-1-AP
1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human DBH (10777-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human DBH (10777-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-Fro detected in
rat brain tissue
Positive IF/ICC detected in
SH-SY5Y cells, HeLa cells
Positive FC (Intra) detected in
SH-SY5Y cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:8000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-FRO
IF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
DBH belongs to the copper type II ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of DBH to norepinephrine. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons(PMID:11857564).The DBH is glycosylated (63--67 kDa) while still in the membrane(PMID:2325165) and it can form a dimer(PMID:8546710).
Various lysates were subjected to SDS PAGE followed by western blot with 10777-1-AP (DBH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 10777-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 10777-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10777-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10777-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 10x lens).
IHC staining of human liver cancer using 10777-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 40x lens).
IF-FRO Figures
IF Staining of rat brain using 10777-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using DBH antibody (10777-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF/ICC Figures
IF Staining of SH-SY5Y using 10777-1-AP
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DBH antibody (10777-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HeLa using 10777-1-AP
Immunofluorescent analysis of HeLa cells using 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC (INTRA) Figures
FC experiment of SH-SY5Y using 10777-1-AP
1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human DBH (10777-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
DBH Polyclonal antibody
Catalog Number
10777-1-AP
Citations
2
Dilutions
WB : 1:1000-1:8000 IHC : 1:50-1:500 IF-FRO : 1:50-1:500 IF/ICC : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
