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Decorin Polyclonal antibody
Decorin Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14667-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, MCF-7 cells, BxPC-3 cells, mouse colon tissue, rat heart tissue, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue, human ovary tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 13 publications below |
| IHC | See 4 publications below |
| IF | See 3 publications below |
Product Information
14667-1-AP targets Decorin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Decorin fusion protein Ag6328 |
| Full Name | decorin |
| Calculated molecular weight | 359 aa, 40 kDa |
| Observed molecular weight | 70 kDa, 45-48 kDa |
| GenBank accession number | BC005322 |
| Gene symbol | DCN |
| Gene ID (NCBI) | 1634 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Decorin antibody 14667-1-AP | Download protocol |
| IHC protocol for Decorin antibody 14667-1-AP | Download protocol |
| IF protocol for Decorin antibody 14667-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Genet Impaired proteoglycan glycosylation, elevated TGF-β signaling, and abnormal osteoblast differentiation as the basis for bone fragility in a mouse model for gerodermia osteodysplastica. | ||
Stem Cell Res Ther Extracellular vesicles produced by bone marrow mesenchymal stem cells attenuate renal fibrosis, in part by inhibiting the RhoA/ROCK pathway, in a UUO rat model. | ||
Sci Rep Cellular response to persistent foot-and-mouth disease virus infection is linked to specific types of alterations in the host cell transcriptome. | ||
J Cell Mol Med Single-cell RNA sequencing analysis to characterize cells and gene expression landscapes in atrial septal defect. | ||
J Proteomics Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation. | ||
Glycoconj J Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Huai-Chin (Verified Customer) (10-24-2022) | Good antibody with only single band. Actual band size is higher than the calculated protein size which may indicated some protein modification.
|
FH balawant (Verified Customer) (07-25-2022) | I have this antibody for my research it is working great in both tissue lysate as well as in cell lysate.
|
FH Iram (Verified Customer) (06-13-2022) | Excellent antibody for western blotting
|
FH Balawant (Verified Customer) (05-08-2022) | I have used this antibody. This antibody is excellent, and I used it for western blotting. The dilution of 1:1000 is functioning well.
|
FH Susmita (Verified Customer) (09-17-2020) | Antibody is working very good for WB
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