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Decorin Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 14667-1-AP

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Con: 27 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Decorin Antibody WB MCF-7 cells 14667-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 14667-1-AP (Decorin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Decorin Antibody IHC human ovary tissue 14667-1-AP

Immunohistochemical of paraffin-embedded human ovary using 14667-1-AP(Decorin antibody) at dilution of 1:50 (under 40x lens)

Decorin Antibody IF BxPC-3 cells 14667-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed BxPC-3 cells using 14667-1-AP(Decorin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, hamster
Positive WB detected in:
MCF-7 cells, mouse heart tissue, BxPC-3 cells, NIH/3T3 cells, mouse colon tissue
Positive IHC detected in:
human ovary tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
BxPC-3 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Decorin fusion protein Ag6328
Full name:
decorin
Calculated molecular weight:
359aa,40 kDa
Observed molecular weight:
70 kDa, 45-48 kDa
GenBank accession number:
BC005322
Gene ID (NCBI):
1634
Gene symbol
DCN
Synonyms
Bone proteoglycan II, CSCD, DCN, Decorin, DSPG2, PG S2, PG40, PGII, PGS2, SLRR1B

Background

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.


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