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Decorin Polyclonal antibody

Decorin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14667-1-AP

Synonyms

Bone proteoglycan II, CSCD, DCN, Decorin, DSPG2, PG S2, PG40, PGII, PGS2, SLRR1B



Tested Applications

Positive WB detected inmouse heart tissue, MCF-7 cells, BxPC-3 cells, mouse colon tissue, rat heart tissue, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human ovary tissue, human skin cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14667-1-AP targets Decorin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Decorin fusion protein Ag6328
Full Name decorin
Calculated molecular weight 359 aa, 40 kDa
Observed molecular weight 70 kDa, 45-48 kDa
GenBank accession numberBC005322
Gene symbol DCN
Gene ID (NCBI) 1634
RRIDAB_2090265
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.

Protocols

Product Specific Protocols
WB protocol for Decorin antibody 14667-1-APDownload protocol
IHC protocol for Decorin antibody 14667-1-APDownload protocol
IF protocol for Decorin antibody 14667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Breast Cancer Res

Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors

Authors - Wei Yang
mouseWB,IF

PLoS Genet

Impaired proteoglycan glycosylation, elevated TGF-β signaling, and abnormal osteoblast differentiation as the basis for bone fragility in a mouse model for gerodermia osteodysplastica.

Authors - Wing Lee Chan
mouseIHC, WB

Cell Cycle

Negative impact of hyperglycaemia on mouse alveolar development.

Authors - Mei-Yao He
ratWB

Stem Cell Res Ther

Extracellular vesicles produced by bone marrow mesenchymal stem cells attenuate renal fibrosis, in part by inhibiting the RhoA/ROCK pathway, in a UUO rat model.

Authors - Zhengzhou Shi
hamsterWB

Sci Rep

Cellular response to persistent foot-and-mouth disease virus infection is linked to specific types of alterations in the host cell transcriptome.

Authors - Lingling Han
humanIHC

J Cell Mol Med

Single-cell RNA sequencing analysis to characterize cells and gene expression landscapes in atrial septal defect.

Authors - Zunzhe Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kenzo (Verified Customer) (08-29-2023)

Antibody is working pretty well for IF on mouse tissue sections.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Liver
FH

Huai-Chin (Verified Customer) (10-24-2022)

Good antibody with only single band. Actual band size is higher than the calculated protein size which may indicated some protein modification.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: used on both mouse and human cell lines
FH

balawant (Verified Customer) (07-25-2022)

I have this antibody for my research it is working great in both tissue lysate as well as in cell lysate.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: colon tissue and Caco2 cells
FH

Iram (Verified Customer) (06-13-2022)

Excellent antibody for western blotting

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: colon cancer cells
FH

Balawant (Verified Customer) (05-08-2022)

I have used this antibody. This antibody is excellent, and I used it for western blotting. The dilution of 1:1000 is functioning well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mice tissue and colon epithelial cells.
FH

Susmita (Verified Customer) (09-17-2020)

Antibody is working very good for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 WB