|Positive WB detected in||mouse heart tissue, MCF-7 cells, BxPC-3 cells, NIH/3T3 cells, mouse colon tissue, rat heart tissue|
|Positive IHC detected in||human skin cancer tissue, human ovary tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||BxPC-3 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14667-1-AP targets Decorin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||hamster, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Decorin fusion protein Ag6328|
|Calculated molecular weight||359 aa, 40 kDa|
|Observed molecular weight||70 kDa, 45-48 kDa|
|GenBank accession number||BC005322|
|Gene ID (NCBI)||1634|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Susmita (Verified Customer) (09-17-2020)
Antibody is working very good for WB