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Decorin Polyclonal antibody

Decorin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14667-1-AP

Synonyms

Bone proteoglycan II, CSCD, DCN, Decorin, DSPG2, PG S2, PG40, PGII, PGS2, SLRR1B



Tested Applications

Positive WB detected inmouse heart tissue, MCF-7 cells, BxPC-3 cells, NIH/3T3 cells, mouse colon tissue, rat heart tissue
Positive IHC detected inhuman skin cancer tissue, human ovary tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14667-1-AP targets Decorin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity hamster, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Decorin fusion protein Ag6328
Full Name decorin
Calculated molecular weight 359 aa, 40 kDa
Observed molecular weight 70 kDa, 45-48 kDa
GenBank accession numberBC005322
Gene symbol DCN
Gene ID (NCBI) 1634
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for Decorin antibody 14667-1-APDownload protocol
IHC protocol for Decorin antibody 14667-1-APDownload protocol
IF protocol for Decorin antibody 14667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Physiol

Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.

Authors - He Wang
IHC

Am J Physiol Lung Cell Mol Physiol

Identification of CAV1 and DCN as potential predictive biomarkers for lung adenocarcinoma.

Authors - Yuanliang Yan
humanWB

JCI Insight

Hypomorphic mutations of TRIP11 cause odontochondrodysplasia.

Authors - Anika Wehrle
mouseIHC, WB

Cell Cycle

Negative impact of hyperglycaemia on mouse alveolar development.

Authors - Mei-Yao He
mouseWB

Pharm Biol

Effects of polysaccharide from Physalis alkekengi var. francheti on liver injury and intestinal microflora in type-2 diabetic mice.

Authors - Xin Zhao
mouseWB

Pharm Biol

Effects of garlic polysaccharide on alcoholic liver fibrosis and intestinal microflora in mice.

Authors - Yuchuan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Susmita (Verified Customer) (09-17-2020)

Antibody is working very good for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 WB