|Positive WB detected in||mouse brain tissue, mouse kidney tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human liver cancer tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:600|
|Sample-dependent, check data in validation data gallery|
10166-1-AP targets DOPA decarboxylase in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DOPA decarboxylase fusion protein Ag0219|
|Full Name||dopa decarboxylase (aromatic L-amino acid decarboxylase)|
|Calculated molecular weight||54 kDa|
|Observed molecular weight||48-50 kDa|
|GenBank accession number||BC008366|
|Gene ID (NCBI)||1644|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Aromatic-L-amino-acid decarboxylase belongs to the pyridoxal-dependent aminotransferase superfamily.DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD).Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.
|Product Specific Protocols|
|WB protocol for DOPA decarboxylase antibody 10166-1-AP||Download protocol|
|IHC protocol for DOPA decarboxylase antibody 10166-1-AP||Download protocol|
|IP protocol for DOPA decarboxylase antibody 10166-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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