DOPA decarboxylase Polyclonal antibody

DOPA decarboxylase Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10166-1-AP

Synonyms

AADC, DDC, DOPA decarboxylase



Tested Applications

Positive WB detected inmouse brain tissue, mouse kidney tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:600
Sample-dependent, check data in validation data gallery

Product Information

10166-1-AP targets DOPA decarboxylase in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOPA decarboxylase fusion protein Ag0219
Full Name dopa decarboxylase (aromatic L-amino acid decarboxylase)
Calculated molecular weight 54 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC008366
Gene symbol DDC
Gene ID (NCBI) 1644
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aromatic-L-amino-acid decarboxylase belongs to the pyridoxal-dependent aminotransferase superfamily.DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD).Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.

Protocols

Product Specific Protocols
WB protocol for DOPA decarboxylase antibody 10166-1-APDownload protocol
IHC protocol for DOPA decarboxylase antibody 10166-1-APDownload protocol
IP protocol for DOPA decarboxylase antibody 10166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

J Chem Neuroanat

Molecular phenotyping of transient postnatal tyrosine hydroxylase neurons in the rat bed nucleus of the stria terminalis.

Authors - David A Carter
humanWB

J Transl Med

Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson's disease.

Authors - Ming Ming
mouseIHC,IF

Toxicol Appl Pharmacol

PMID: 21703292

Authors - Fox Donald A DA
mouseIF

Nature

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Authors - Hao Qian