Tested Applications
| Positive WB detected in | SGC-7901 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85709-1-RR targets DDX10 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag20766 Product name: Recombinant human DDX10 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 100-431 aa of BC091521 Sequence: IGLALQGKDVLGAAKTGSGKTLAFLVPVLEALYRLQWTSTDGLGVLIISPTRELAYQTFEVLRKVGKNHDFSAGLIIGGKDLKHEAERINNINILVCTPGRLLQHMDETVSFHATDLQMLVLDEADRILDMGFADTMNAVIENLPKKRQTLLFSATQTKSVKDLARLSLKNPEYVWVHEKAKYSTPATLEQNYIVCELQQKISVLYSFLRSHLKKKSIVFFSSCKEVQYLYRVFCRLRPGVSILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPAVNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEKAMVQQLL Predict reactive species |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 |
| Calculated Molecular Weight | 875 aa, 101 kDa |
| Observed Molecular Weight | 101-110 kDa |
| GenBank Accession Number | BC091521 |
| Gene Symbol | DDX10 |
| Gene ID (NCBI) | 1662 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13206 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DDX10, DEAD-box helicase 10, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Fusion of this gene and the nucleoporin gene NUP98 is found in leukemogenesis (PMID:20339440).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX10 antibody 85709-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
