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  • Featured Product
  • KD/KO Validated

DDX24 Polyclonal antibody

DDX24 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15769-1-AP

Synonyms

DDX24, DEAD box protein 24

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX24 Polyclonal antibody (15769-1-AP)

    WB analysis using 15769-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 15769-1-AP (DDX24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DDX24 Polyclonal antibody (15769-1-AP)

    WB analysis using 15769-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15769-1-AP (DDX24 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DDX24 Polyclonal antibody (15769-1-AP)

    IF Staining of HeLa using 15769-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX24 antibody (15769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHT-29 cells, HeLa cells, THP-1 cells, Y79 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15769-1-AP targets DDX24 in WB, RIP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX24 fusion protein Ag8407
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC008847
Gene Symbol DDX24
Gene ID (NCBI) 57062
RRIDAB_2246003
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX24 is a family member of Asp-Glu-Ala-Asp (DEAD) box containing RNA helicases, and DEAD-box RNA helicases are characterized by a conserved DEAD motif. DDX24 was associated with cancer development, viral infection,and vascular malformation.

Protocols

Product Specific Protocols
WB protocol for DDX24 antibody 15769-1-APDownload protocol
IF protocol for DDX24 antibody 15769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,RIP

Cancer Res

RNA helicase DDX24 stabilizes LAMB1 to promote hepatocellular carcinoma progression.

Authors - Hong Shan
  • KD Validated
View Article
humanIF

Mol Cell

1The RNA helicase DDX39A binds a conserved structure in chikungunya virus RNA to control infection

Authors - Iulia Tapescu
View Article