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DDX54 Polyclonal antibody

DDX54 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 26894-1-AP

Synonyms

DDX54, DEAD box protein 54, DEAD box RNA helicase 97 kDa, DP97

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DDX54 Polyclonal antibody (26894-1-AP)

    WB analysis of HeLa using 26894-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26894-1-AP (DDX54 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using DDX54 Polyclonal antibody (26894-1-AP)

    IF Staining of PC-3 using 26894-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DDX54 antibody (26894-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26894-1-AP targets DDX54 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX54 fusion protein Ag25289
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Calculated Molecular Weight 98 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC156669
Gene Symbol DDX54
Gene ID (NCBI) 79039
RRIDAB_2880671
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX54, also named as ATP-dependent RNA helicase DDX54, is a 881 amino acid protein, which contains 1 helicase ATP-binding domain and belongs to the DEAD box helicase family. DDX54/DBP10 subfamily. DDX54 localizes in nucleus and Interacts in a hormone-dependent manner with nuclear receptors. DDX54 has RNA-dependent ATPase activity and represses the transcriptional activity of nuclear receptors.

Protocols

Product Specific Protocols
WB protocol for DDX54 antibody 26894-1-APDownload protocol
IF protocol for DDX54 antibody 26894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

bioRxiv

Nucleolar dynamics are determined by the ordered assembly of the ribosome

Authors - Jessica Sheu-Gruttadauria
  • KD Validated
View Article