Desmin Polyclonal antibody

Desmin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22205-1-AP

Synonyms

CMD1I, CSM1, CSM2, DES, desmin



Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22205-1-AP is Desmin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Desmin fusion protein Ag17525
Full Name desmin
Calculated molecular weight 470 aa, 54 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC032116
Gene symbol DES
Gene ID (NCBI) 1674
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Protocols

Product Specific Protocols
WB protocol for Desmin antibody 22205-1-APDownload protocol
IHC protocol for Desmin antibody 22205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols