• Featured Product
  • KD/KO Validated

DGCR8 N-terminal Polyclonal antibody

DGCR8 N-terminal Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 25835-1-AP

Synonyms

C22orf12, DGCR8, DGCRK6, Gy1



Tested Applications

Positive WB detected inHeLa cells, A431 cells
Positive IP detected inA431 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25835-1-AP is DGCR8 N-terminal Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DGCR8 N-terminal fusion protein Ag22988
Full Name DiGeorge syndrome critical region gene 8
Calculated molecular weight 773 aa, 86 kDa
Observed molecular weight 88-100 kDa
GenBank accession numberBC078147
Gene symbol DGCR8
Gene ID (NCBI) 54487
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.

Protocols

Product Specific Protocols
WB protocol for DGCR8 N-terminal antibody 25835-1-APDownload protocol
IF protocol for DGCR8 N-terminal antibody 25835-1-APDownload protocol
IP protocol for DGCR8 N-terminal antibody 25835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols