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DGKB Polyclonal antibody

DGKB Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24320-1-AP

Synonyms

90 kDa diacylglycerol kinase, DAG kinase beta, DAGK2, DGK, DGK BETA, DGKB, Diacylglycerol kinase beta, Diglyceride kinase beta, KIAA0718

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DGKB Polyclonal antibody (24320-1-AP)

    WB analysis of HeLa using 24320-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24320-1-AP (DGKB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DGKB Polyclonal antibody (24320-1-AP)

    IHC staining of human breast cancer using 24320-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24320-1-AP (DGKB Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DGKB Polyclonal antibody (24320-1-AP)

    IHC staining of human breast cancer using 24320-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24320-1-AP (DGKB Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24320-1-AP is DGKB Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DGKB fusion protein Ag19809
Full Name diacylglycerol kinase, beta 90kDa
Calculated Molecular Weight 804 aa, 91 kDa
Observed Molecular Weight 91 kDa
GenBank Accession NumberBC105005
Gene Symbol DGKB
Gene ID (NCBI) 1607
RRIDAB_2879492
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DGKB(Diacylglycerol kinase beta) is also named as DAGK2, KIAA0718 , 90 kDa diacylglycerol kinase and belongs to the eukaryotic diacetylglycerol kinase family. It is a key enzyme in lipid metabolism that functions to reintroduce diacylglycerol formed from the hydrolysis of phospholipids into the biosynthetic pathway. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 87 kDa and 91 kDa.

Protocols

Product Specific Protocols
WB protocol for DGKB antibody 24320-1-APDownload protocol
IHC protocol for DGKB antibody 24320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols