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DGKK-Specific Polyclonal antibody

DGKK-Specific Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20090-1-AP

Synonyms

142 kDa diacylglycerol kinase, DAG kinase kappa, DGK kappa, DGKK, Diacylglycerol kinase kappa, diacylglycerol kinase, kappa, Diglyceride kinase kappa

Validation Data Gallery

  • WB analysis of human testis using 20090-1-AP

    WB analysis of human testis using 20090-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 20090-1-AP

    IF Staining of HepG2 using 20090-1-AP

    Immunofluorescent analysis of HepG2 cells, using DGKK antibody 20090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inhuman testis tissue, HuH-7 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20090-1-AP targets DGKK-Specific in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name diacylglycerol kinase, kappa
Calculated molecular weight 142 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberNM_001013742
Gene symbol DGKK
Gene ID (NCBI) 139189
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DGKK Belongs to the eukaryotic diacylglycerol kinase family. DGKK phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). DGKK catalyze the reaction: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. The antibody is specific to DGKK.

Protocols

Product Specific Protocols
WB protocol for DGKK-Specific antibody 20090-1-APDownload protocol
IF protocol for DGKK-Specific antibody 20090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols