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DGKK-Specific Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 20090-1-AP

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Con: 45 μg/150 μl

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Tested Applications

Applications:
WB, IF, ELISA
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Images See all

DGKK-Specific Antibody WB human testis tissue 20090-1-AP

human testis tissue were subjected to SDS PAGE followed by western blot with 20090-1-AP(DGKK-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

DGKK-Specific Antibody IF HepG2 cells 20090-1-AP

Immunofluorescent analysis of HepG2 cells, using DGKK antibody 20090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Species specificity:
human
Positive WB detected in:
human testis tissue, HuH-7 cells
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
diacylglycerol kinase, kappa
Calculated molecular weight:
142 kDa
Observed molecular weight:
135-140 kDa
GenBank accession number:
NM_001013742
Gene ID (NCBI):
139189
Gene symbol
DGKK
Synonyms
142 kDa diacylglycerol kinase, DAG kinase kappa, DGK kappa, DGKK, Diacylglycerol kinase kappa, diacylglycerol kinase, kappa, Diglyceride kinase kappa

Background

DGKK Belongs to the eukaryotic diacylglycerol kinase family. DGKK phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). DGKK catalyze the reaction: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. The antibody is specific to DGKK.


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