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  • Western Blot (WB) analysis of human testis tissue using DGKK-Specific Polyclonal antibody (20090-1-AP)

    WB analysis of human testis using 20090-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using DGKK-Specific Polyclonal antibody (20090-1-AP)

    WB analysis of human testis using 20090-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HuH-7 cells using DGKK-Specific Polyclonal antibody (20090-1-AP)

    WB analysis of HuH-7 using 20090-1-AP

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DGKK-Specific Polyclonal antibody (20090-1-AP)

    IF Staining of HepG2 using 20090-1-AP

    Immunofluorescent analysis of HepG2 cells, using DGKK antibody 20090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

DGKK-Specific Polyclonal antibody

DGKK-Specific Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20090-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

DGKK, EC:2.7.1.107, DGK-kappa, DGK kappa, DAG kinase kappa

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inhuman testis tissue, HuH-7 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20090-1-AP targets DGKK-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide Predict reactive species
Full Name diacylglycerol kinase, kappa
Calculated Molecular Weight 142 kDa
Observed Molecular Weight 135-140 kDa
GenBank Accession NumberNM_001013742
Gene Symbol DGKK
Gene ID (NCBI) 139189
RRIDAB_10666998
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5KSL6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DGKK Belongs to the eukaryotic diacylglycerol kinase family. DGKK phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). DGKK catalyze the reaction: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. The antibody is specific to DGKK.

Protocols

Product Specific Protocols
WB protocol for DGKK-Specific antibody 20090-1-APDownload protocol
IF protocol for DGKK-Specific antibody 20090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols