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  • KD/KO Validated

DGKQ Polyclonal antibody

DGKQ Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17885-1-AP

Synonyms

DAG kinase theta, DAGK, DAGK4, DAGK7, DGK theta, DGKQ, Diacylglycerol kinase theta, Diglyceride kinase theta



Tested Applications

Positive WB detected inmouse brain tissue, human placenta tissue, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human cervix tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17885-1-AP targets DGKQ in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DGKQ fusion protein Ag11938
Full Name diacylglycerol kinase, theta 110kDa
Calculated Molecular Weight 942 aa, 101 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC063801
Gene Symbol DGKQ
Gene ID (NCBI) 1609
RRIDAB_2091305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DGKQ antibody 17885-1-APDownload protocol
IHC protocol for DGKQ antibody 17885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.

Authors - Jingjing Zhang
  • KO Validated
WB

J Sci Food Agric

Neonatal resveratrol administration promotes skeletal muscle growth and insulin sensitivity in intrauterine growth-retarded suckling piglets associated with activation of FGF21-AMPKα pathway

Authors - Guangyi Bai