Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60028-1-Ig targets DHCR24 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0756 Product name: Recombinant human DHCR24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 217-516 aa of BC004375 Sequence: SCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGLEAICAKFTHESQRQENHFVEGLLYSLDEAVIMTGVMTDEAEPSKLNSIGNYYKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCPFILPSQPGLVHPKGNEAELYIDIGAYGEPRVKHFEARSCMRQLEKFVRSVHGFQMLYADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAARH Predict reactive species |
| Full Name | 24-dehydrocholesterol reductase |
| Calculated Molecular Weight | 60 kDa, 516 aa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC004375 |
| Gene Symbol | DHCR24 |
| Gene ID (NCBI) | 1718 |
| RRID | AB_874115 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15392 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The dhcr24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DHCR24 antibody 60028-1-Ig | Download protocol |
| WB protocol for DHCR24 antibody 60028-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
DHCR24 Monoclonal antibody (2F6E11), catalog number 60028-1-Ig, has 2 total citations. These citations appear in Cell Reports and iScience. The research fields associated with these citations include sterol synthesis, cholesterol biosynthesis, and hepatocellular carcinoma (HCC) progression, with applications in Western blot for both human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Rep Post-translational switch of DHCR24 acetylation sustains sterol synthesis and promotes HCC via the 7-ketocholesterol/p62 axis. | ||
iScience NAT10 drives hepatocellular carcinoma progression through SQLE-mediated cholesterol biosynthesis and is targetable by remodelin. |
