Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25746-1-AP targets DIS3L in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22689 Product name: Recombinant human DIS3L protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 727-971 aa of BC022089 Sequence: LLMAAISKDKKMEIKGNLFSNKDLEELCRHINNRNQAAQHSQKQSTELFQCMYFKDKDPATEERCISDGVIYSIRTNGVLLFIPRFGIKGAAYLKNKDGLVISCGPDSCSEWKPGSLQRFQNKITSTTTDGESVTFHLFDHVTVRISIQASRCHSDTIRLEIISNKPYKIPNTELIHQSSPLLKSELVKEVTKSVEEAQLAQEVKVNIIQEEYQEYRQTKGRSLYTLLEEIRDLALLDVSNNYGI Predict reactive species |
| Full Name | DIS3 mitotic control homolog (S. cerevisiae)-like |
| Observed Molecular Weight | 121 kDa |
| GenBank Accession Number | BC022089 |
| Gene Symbol | DIS3L |
| Gene ID (NCBI) | 115752 |
| RRID | AB_2880222 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TF46 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for DIS3L antibody 25746-1-AP | Download protocol |
| WB protocol for DIS3L antibody 25746-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DIS3L Polyclonal antibody (Cat# 25746-1-AP) has 3 total citations. These appear in Cell Reports, RNA, and Life Science Alliance. The cited research spans RNA biology (circular RNA processing and exosome complex function), developmental biology, and virology/immunology (SARS-CoV-2 replication). All three studies used the antibody for Western blot applications in human or mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Rep Cytoplasmic DIS3 is an exosome-independent endoribonuclease with catalytic activity toward circular RNAs | ||
RNA DIS3L, cytoplasmic exosome catalytic subunit, is essential for development but not cell viability in mice | ||
Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication |
Reviews
What customers say
One customer rated this antibody 5 stars for Western Blot on mESCs at a 1:1000 dilution (lot 00024047). The reviewer noted that the antibody specifically recognizes Dis3L, indicating strong specificity and performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tsimafei (Verified Customer) (08-07-2024) | Antibody specifically recognize Dis3L
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