SAP102 Antibody

SAP102 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18036-1-AP

Synonyms

Disks large homolog 3, DLG3, KIAA1232, MRX, MRX90, NE Dlg, NEDLG, Neuroendocrine DLG, SAP 102, SAP102, Synapse associated protein 102, XLMR



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:6000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

18036-1-AP targets SAP102 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAP102 fusion protein Ag12658
Full Name discs, large homolog 3 (Drosophila)
Calculated molecular weight 817aa,90 kDa
Observed molecular weight 100 kDa
GenBank accession number BC093864
Gene symbol DLG3
Gene ID (NCBI) 1741
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synapse-associated protein 102 (SAP102), also known as DLG3, is a MAGUK that is highly expressed early in development and mediates receptor trafficking during synaptogenesis. The importance of SAP102 function in brain development is demonstrated by the fact that SAP102 mutations have been identified in human patients with nonsyndromic X-linked mental retardation (XLMR).SAP102 has critical roles in early cortical synapse development.

Protocols

Product Specific Protocols
WB protocol for SAP102 antibody 18036-1-APDownload protocol
IHC protocol for SAP102 antibody 18036-1-APDownload protocol
IP protocol for SAP102 antibody 18036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Clin Exp Pharmacol Physiol

High expression of DLG3 is associated with decreased survival from breast cancer.

Authors - Jie Liu