• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DLK2 Polyclonal antibody

DLK2 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 15680-1-AP

Synonyms

DLK 2, DLK2, EGF like protein 9, EGFL9, Protein delta homolog 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using DLK2 Polyclonal antibody (15680-1-AP)

    WB analysis of mouse brain using 15680-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15680-1-AP (DLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using DLK2 Polyclonal antibody (15680-1-AP)

    WB analysis of human liver using 15680-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15680-1-AP (DLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DLK2 Polyclonal antibody (15680-1-AP)

    IP experiment of mouse brain using 15680-1-AP

    IP result of anti-DLK2 (IP:15680-1-AP, 4ug; Detection:15680-1-AP 1:500) with mouse brain tissue lysate 5600 ug.

Tested Applications

Positive WB detected inmouse brain tissue, human liver tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15680-1-AP targets DLK2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DLK2 fusion protein Ag8170
Full Name delta-like 2 homolog (Drosophila)
Calculated Molecular Weight383aa,41 kDa; 204aa,21 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC006425
Gene Symbol DLK2
Gene ID (NCBI) 65989
RRIDAB_2092804
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DLK2 antibody 15680-1-APDownload protocol
IP protocol for DLK2 antibody 15680-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Different Expression Levels of DLK2 Inhibit NOTCH Signaling and Inversely Modulate MDA-MB-231 Breast Cancer Tumor Growth In Vivo.

Authors - Ana-Isabel Naranjo
View Article
mouse

Sci Rep

DLK proteins modulate NOTCH signaling to influence a brown or white 3T3-L1 adipocyte fate.

Authors - María-Luisa Nueda
View Article
ratWB

Tissue Cell

Delta like Non-Canonical Notch Ligand 2 inhibits chondrogenic differentiation and cell proliferation of bone marrow mesenchymal stem cells through the Notch1 signaling pathway

Authors - Yanan Chen
View Article