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  • Featured Product
  • KD/KO Validated

DMAP1 Polyclonal antibody

DMAP1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10411-1-AP

Synonyms

DMAP1, DNMAP1, DNMT1 associated protein 1, DNMTAP1, EAF2, KIAA1425, SWC4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DMAP1 Polyclonal antibody (10411-1-AP)

    WB analysis using 10411-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using DMAP1 Polyclonal antibody (10411-1-AP)

    WB analysis of NIH/3T3 using 10411-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using DMAP1 Polyclonal antibody (10411-1-AP)

    WB analysis of COLO 320 using 10411-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DMAP1 Polyclonal antibody (10411-1-AP)

    WB analysis of HeLa using 10411-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DMAP1 Polyclonal antibody (10411-1-AP)

    WB analysis of HeLa using 10411-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using DMAP1 Polyclonal antibody (10411-1-AP)

    WB analysis of COLO 320 using 10411-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using DMAP1 Polyclonal antibody (10411-1-AP)

    IP experiment of COLO 320 using 10411-1-AP

    IP result of anti-DMAP1 (IP:10411-1-AP, 3ug; Detection:10411-1-AP 1:300) with COLO 320 cells lysate 2500ug.

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, NIH/3T3 cells
Positive IP detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10411-1-AP targets DMAP1 in WB, RIP, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DMAP1 fusion protein Ag0665
Full Name DNA methyltransferase 1 associated protein 1
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 53-60 kDa
GenBank Accession NumberBC002855
Gene Symbol DMAP1
Gene ID (NCBI) 55929
RRIDAB_2277167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2). The antibody reacts specifically with DMAP1 protein.

Protocols

Product Specific Protocols
WB protocol for DMAP1 antibody 10411-1-APDownload protocol
IP protocol for DMAP1 antibody 10411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

An RNAi screen of chromatin proteins identifies Tip60-p400 as a regulator of embryonic stem cell identity.

Authors - Thomas G Fazzio
  • KD Validated
View Article
mouse

Nat Struct Mol Biol

R loops regulate promoter-proximal chromatin architecture and cellular differentiation.

Authors - Poshen B Chen
View Article
humanWB

EMBO Mol Med

Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis.

Authors - Jinbeom Heo
View Article
mouseWB

Elife

Hdac6 regulates Tip60-p400 function in stem cells.

Authors - Chen Poshen B PB
View Article
mouseWB

Cell Rep

Sirt1 Regulates DNA Methylation and Differentiation Potential of Embryonic Stem Cells by Antagonizing Dnmt3l.

Authors - Jinbeom Heo
View Article
mouseChIP,RIP

Psychiatry Investig

m6A Methyltransferase METTL3 Reduces Hippocampal Neuron Apoptosis in a Mouse Model of Autism Through the MALAT1/SFRP2/Wnt/β-catenin Axis

Authors - Yue Ming
View Article