|Positive WB detected in||COLO 320 cells, HeLa cells, NIH/3T3 cells|
|Positive IP detected in||COLO 320 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
10411-1-AP targets DMAP1 in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DMAP1 fusion protein Ag0665|
|Full Name||DNA methyltransferase 1 associated protein 1|
|Calculated molecular weight||53 kDa|
|Observed molecular weight||53-60 kDa|
|GenBank accession number||BC002855|
|Gene ID (NCBI)||55929|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identiﬁed as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2). The antibody reacts specifically with DMAP1 protein.
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Hdac6 regulates Tip60-p400 function in stem cells.
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Nat Struct Mol Biol
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An RNAi screen of chromatin proteins identifies Tip60-p400 as a regulator of embryonic stem cell identity.