DNAH6 Polyclonal antibody

DNAH6 Polyclonal Antibody for IHC, ELISA

Cat No. 18080-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

Ciliary dynein heavy chain 6, DNAH6, Dnahc6, DNHL1, Dynein heavy chain 6, axonemal, HL 2, HL2, KIAA1697

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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Filter: AllIHC

IHC staining of mouse brain using 18080-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18080-1-AP (DNAH6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 18080-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18080-1-AP (DNAH6 antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 18080-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18080-1-AP (DNAH6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 18080-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18080-1-AP (DNAH6 antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in

human small intestine tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18080-1-AP targets DNAH6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12774 Product name: Recombinant human DNAH6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-408 aa of BC104884 Sequence: MTYTEQASKRHYCMRLTCFIRLNDYLIENTMHILTVNAVNSLLNHLTDKLKRTPSADVIQKWITEEKPEVPDKKGTLMVEKQEEDESLIPMFLTELMLTVQSLLFEPSLEDFLDGILGAVNHCQNTVLSVPNLVPDSYFDAFTSPYINNKLEGKTCGTGPSLAAVFEDDKNFHTIISQIKETIQAAFESARIYAATFEKFQIFFKENESLDLQALKLQEPDINFFSEQLEKYHKQHKDAVALRPTRNVGLLLIDTRLLREKLIPSPLRCLEVLNFMLPRQSKKKVDAIIFEAQDAEYKLEFVPTTTTEYVHSLLFLDEIQERIESLEDEGNIVTQMYKLMEQYQVPTPPEDFAVFATMKPSIVAVRNAIDKSVGDRESSIKQFCVHLGSDLEELNNEVNEVKLQAQVS Predict reactive species
Full Name	dynein, axonemal, heavy chain 6
Calculated Molecular Weight	475 kDa
GenBank Accession Number	BC104884
Gene Symbol	DNAH6
Gene ID (NCBI)	1768
RRID	AB_2878493
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9C0G6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAH6(Dynein heavy chain 6, axonemal) is also named as DNAHC6, DNHL1, HL2, KIAA1697 and belongs to the dynein heavy chain family.Dyneins are microtubule-associated motor protein complexes composed of several heavy, light, and intermediate chains(PMID:8812413). It is a force generating protein of respiratory cilia and produces force towards the minus ends of microtubules.This antibody is specific to DNAH6.

Protocols

Product Specific Protocols
IHC protocol for DNAH6 antibody 18080-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human,mouse	IHC	Mol Psychiatry FXR1 regulation of parvalbumin interneurons in the prefrontal cortex is critical for schizophrenia-like behaviors. Authors - Minjie Shen View Article
human	WB,IF	Elife DNAH3 deficiency causes flagellar inner dynein arm loss and male infertility in humans and mice Authors - Xiang Wang View Article