DNAI2 Polyclonal antibody

DNAI2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17533-1-AP

Synonyms

CILD9, DNAI2



Tested Applications

Positive WB detected inmouse lung tissue, HeLa cells, mouse ovary tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17533-1-AP targets DNAI2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAI2 fusion protein Ag11585
Full Name dynein, axonemal, intermediate chain 2
Calculated molecular weight 593 aa, 67 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC039582
Gene symbol DNAI2
Gene ID (NCBI) 64446
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAI2 antibody 17533-1-APDownload protocol
IHC protocol for DNAI2 antibody 17533-1-APDownload protocol
IP protocol for DNAI2 antibody 17533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Ann Hum Genet

DNAH17 is associated with asthenozoospermia and multiple morphological abnormalities of sperm flagella.

Authors - Yanwei Sha
mouseIF

J Mol Cell Biol

Production of transgenic mice by random recombination of targeted genes in female germline stem cells.

Authors - Zhang Yong Y
mouseWB,IF,IP

Elife

ZMYND10 functions in a chaperone relay during axonemal dynein assembly.

Authors - Girish R Mali