DNAI2 Polyclonal antibody

DNAI2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17533-1-AP

Synonyms

CILD9, DNAI2



Tested Applications

Positive WB detected inmouse ovary tissue, HeLa cells, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17533-1-AP targets DNAI2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAI2 fusion protein Ag11585
Full Name dynein, axonemal, intermediate chain 2
Calculated Molecular Weight 593 aa, 67 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC039582
Gene Symbol DNAI2
Gene ID (NCBI) 64446
RRIDAB_2096670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DNAI2 antibody 17533-1-APDownload protocol
IHC protocol for DNAI2 antibody 17533-1-APDownload protocol
IP protocol for DNAI2 antibody 17533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF,IP

Elife

ZMYND10 functions in a chaperone relay during axonemal dynein assembly.

Authors - Girish R Mali
mouseIF

J Mol Cell Biol

Production of transgenic mice by random recombination of targeted genes in female germline stem cells.

Authors - Zhang Yong Y
ratWB,IF

Front Cell Dev Biol

PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia

Authors - Tingting Zhang
mouseWB

Development

CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility

Authors - Keisuke Shimada
mouseWB

Exp Anim

The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum.

Authors - Haruhiko Miyata
humanWB

Ann Hum Genet

DNAH17 is associated with asthenozoospermia and multiple morphological abnormalities of sperm flagella.

Authors - Yanwei Sha