Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, mouse heart tissue, HepG2 cells, rat liver tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15484-1-AP targets DNAJB11 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7832 Product name: Recombinant human DNAJB11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-358 aa of BC001144 Sequence: GRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQSSHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAREGIKQLLKQGSVQKVYNGLQGY Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily B, member 11 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC001144 |
| Gene Symbol | DNAJB11 |
| Gene ID (NCBI) | 51726 |
| RRID | AB_2094400 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBS4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAJB11 antibody 15484-1-AP | Download protocol |
| IHC protocol for DNAJB11 antibody 15484-1-AP | Download protocol |
| IP protocol for DNAJB11 antibody 15484-1-AP | Download protocol |
| WB protocol for DNAJB11 antibody 15484-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ERdj3 antibody (Cat# 15484-1-AP) has 26 citations published in journals including Science, Cell, Molecular Cell, EMBO Journal, Cell Reports, Journal of Biological Chemistry, FASEB Journal, and Lab Investigation. Research fields span endoplasmic reticulum stress, unfolded protein response, molecular chaperone biology, proteostasis, viral replication, cancer, kidney disease, and neurodegeneration. Applications were primarily Western blot and immunoprecipitation in human, mouse, and rat samples.
| Species | Application | Title |
|---|---|---|
Cell Defining Hsp70 Subnetworks in Dengue Virus Replication Reveals Key Vulnerability in Flavivirus Infection.
| ||
Mol Cell Differentiation Drives Widespread Rewiring of the Neural Stem Cell Chaperone Network. | ||
Cell Death Dis Shutdown of ER-associated degradation pathway rescues functions of mutant iduronate 2-sulfatase linked to mucopolysaccharidosis type II.
| ||
Cell Mol Biol Lett Exosomal DNAJB11 promotes the development of pancreatic cancer by modulating the EGFR/MAPK pathway
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