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  • KD/KO Validated

DNAJB11 Polyclonal antibody

DNAJB11 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15484-1-AP

Synonyms

ABBP 2, ABBP2, APOBEC1 binding protein 2, DJ9, DnaJ protein homolog 9, DNAJB11, EDJ, ER associated DNAJ, ER associated dnaJ protein 3, ERdj3, ERj3, ERj3p, hDj 9, hDj9, HEDJ, Human DnaJ protein 9, PRO1080, PWP1 interacting protein 4, UNQ537, UNQ537/PRO1080



Tested Applications

Positive WB detected inHeLa cells, mouse heart tissue, mouse liver tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15484-1-AP targets DNAJB11 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB11 fusion protein Ag7832
Full Name DnaJ (Hsp40) homolog, subfamily B, member 11
Calculated molecular weight 41 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC001144
Gene symbol DNAJB11
Gene ID (NCBI) 51726
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DNAJB11 antibody 15484-1-APDownload protocol
IHC protocol for DNAJB11 antibody 15484-1-APDownload protocol
IF protocol for DNAJB11 antibody 15484-1-APDownload protocol
IP protocol for DNAJB11 antibody 15484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Immunol

Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response.

Authors - Paloma Gómez-Fernández
human

Sci Rep

PERK Signaling Regulates Extracellular Proteostasis of an Amyloidogenic Protein During Endoplasmic Reticulum Stress.

Authors - Isabelle C Romine
humanWB

J Biol Chem

Modulation of calreticulin expression reveals a novel exosome mediated mechanism of Z variant alpha-1 antitrypsin disposal.

Authors - Nazli Khodayari
mouseWB

Korean J Physiol Pharmacol

Curcumin modulates the apolipoprotein B mRNA editing by coordinating the expression of cytidine deamination to uridine editosome components in primary mouse hepatocytes.

Authors - Pan He
humanWB

J Biomol Screen

A high-throughput cell-based gaussia luciferase reporter assay for identifying modulators of fibulin-3 secretion.

Authors - Hulleman John D JD
humanWB,IF

J Cell Biochem

Erdj3 has an essential role for Z variant Alpha-1-Antitrypsin Degradation.

Authors - Nazli Khodayari
  • KD Validated