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DNAJC6/AUXILIN Polyclonal antibody

DNAJC6/AUXILIN Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21941-1-AP

Synonyms

AUXILIN, DJC6, DNAJC6, DNAJC6/AUXILIN, KIAA0473

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJC6/AUXILIN Polyclonal antibody (21941-1-AP)

    WB analysis using 21941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DNAJC6/AUXILIN Polyclonal antibody (21941-1-AP)

    WB analysis of HeLa using 21941-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DNAJC6/AUXILIN Polyclonal antibody (21941-1-AP)

    WB analysis of human brain using 21941-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DNAJC6/AUXILIN Polyclonal antibody (21941-1-AP)

    IP experiment of mouse brain using 21941-1-AP

    IP result of anti-DNAJC6/AUXILIN (IP:21941-1-AP, 4ug; Detection:21941-1-AP 1:2000) with mouse brain tissue lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using DNAJC6/AUXILIN Polyclonal antibody (21941-1-AP)

    IHC staining of human brain using 21941-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21941-1-AP (DNAJC6/AUXILIN antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using DNAJC6/AUXILIN Polyclonal antibody (21941-1-AP)

    IHC staining of human brain using 21941-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21941-1-AP (DNAJC6/AUXILIN antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNAJC6/AUXILIN Polyclonal antibody (21941-1-AP)

    IF Staining of HeLa using 21941-1-AP

    Immunofluorescent analysis of HeLa cells using 21941-1-AP (DNAJC6/AUXILIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, human brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21941-1-AP is DNAJC6/AUXILIN Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC6/AUXILIN fusion protein Ag16612
Full Name DnaJ (Hsp40) homolog, subfamily C, member 6
Calculated Molecular Weight 970 aa, 106 kDa
Observed Molecular Weight 100-120 kDa, 86 kDa
GenBank Accession NumberBC109279
Gene Symbol DNAJC6
Gene ID (NCBI) 9829
RRIDAB_2878951
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJC6, also named AUXILIN, belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. It is a neuronal protein that functions specifically in the pathway of clathrin-mediated endocytosis. It shares homology with GAK, and the two proteins act as cochaperones to support the HSC70 -dependent clathrin uncoating of clathrin-coated vesicles (PMID: 2016009). DNAJC6 is expressed in various brain regions, including cerebellum, corpus callosum, cortex, striatum, brainstem, pons, putamen, spinal cord and substantia nigra (PMID: 23211418).

Protocols

Product Specific Protocols
WB protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
IHC protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
IF protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
IP protocol for DNAJC6/AUXILIN antibody 21941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Sci Rep

Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.

Authors - Ermes Candiello
View Article
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem
View Article