DOCK4 Polyclonal antibody

DOCK4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21861-1-AP

Synonyms

dedicator of cytokinesis 4, DOCK4, KIAA0716



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHeLa cells, HEK-293T cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:150-1:600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21861-1-AP is DOCK4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOCK4 fusion protein Ag16516
Full Name dedicator of cytokinesis 4
Calculated molecular weight 2011 aa, 230 kDa
Observed molecular weight 225 kDa
GenBank accession numberBC117689
Gene symbol DOCK4
Gene ID (NCBI) 9732
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DOCK4, originally identified as a product of a gene which is deleted during tumor progression, is a member of DOCK180 family proteins. Dock4 has been found recently to be associated with several neuropsychiatric diseases, including autism, dyslexia, and schizophrenia. Multiple studies in fibroblasts then confirmed that Dock4 is capable of controlling cell migration by transducing several upstream signals, such as Wnt, platelet-derived growth factor, and RhoG, toward activation of Rac1. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers.

Protocols

Product Specific Protocols
WB protocol for DOCK4 antibody 21861-1-APDownload protocol
IHC protocol for DOCK4 antibody 21861-1-APDownload protocol
IF protocol for DOCK4 antibody 21861-1-APDownload protocol
IP protocol for DOCK4 antibody 21861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Proc Natl Acad Sci U S A

Up-regulated cytotrophoblast DOCK4 contributes to over-invasion in placenta accreta spectrum.

Authors - Leah McNally