DOCK7 Polyclonal antibody
DOCK7 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 13000-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, mouse brain tissue, human brain tissue, mouse ovary tissue, Jurkat cells, HeLa cells, HepG2 cells, K-562 cells, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
13000-1-AP targets DOCK7 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DOCK7 fusion protein Ag3671 |
| Full Name | dedicator of cytokinesis 7 |
| Calculated Molecular Weight | 2109 aa, 239 kDa |
| Observed Molecular Weight | 238-243 kDa |
| GenBank Accession Number | BC016392 |
| Gene Symbol | DOCK7 |
| Gene ID (NCBI) | 85440 |
| RRID | AB_10646476 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DOCK 7 (dedicator of cytokinesis 7), also known as ZIR2, is a member of the DOCK180-related protein superfamily. Expressed mainly in neuronal cells, DOCK 7 is a guanine nucleotide exchange factor (GEF) for small GTPases, Rac1 and Cdc42, which are the major regulators of actin cytoskeleton. Multiple isoforms of DOCK 7 exist due to alternative splicing events. The DOCK 7 antibody (13000-1-AP) can recognize all the isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DOCK7 antibody 13000-1-AP | Download protocol |
| IF protocol for DOCK7 antibody 13000-1-AP | Download protocol |
| IP protocol for DOCK7 antibody 13000-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Biol Cell The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα. | ||
Nat Cell Biol EGFR ligand shifts the role of EGFR from oncogene to tumour suppressor in EGFR-amplified glioblastoma by suppressing invasion through BIN3 upregulation. | ||
Neurobiol Dis The Warburg micro syndrome protein RAB3GAP1 modulates neuronal morphogenesis and interacts with axon elongation end ER-Golgi trafficking factors | ||
Phytomedicine Protective effect of ethyl ferulate against hypoxic injury in retinal cells and retinal neovascularization in an oxygen-induced retinopathy model |
