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DRD2 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 55084-1-AP

Featured Product KD/KO validated

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Con: 63 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

DRD2 Antibody WB HEK-293 cells 55084-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 55084-1-AP( DRD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

DRD2 Antibody IP SH-SY5Y cells 55084-1-AP

IP Result of anti-DRD2 (IP:55084-1-AP, 4ug; Detection:55084-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

DRD2 Antibody IHC mouse brain tissue 55084-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 55084-1-AP( DRD2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

DRD2 Antibody IHC mouse brain tissue 55084-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 55084-1-AP( DRD2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells, SH-SY5Y cells
Positive IP detected in:
SH-SY5Y cells
Positive IHC detected in:
mouse brain tissue, human brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
dopamine receptor D2
Calculated molecular weight:
51 kDa
Observed molecular weight:
51 kDa
GenBank accession number:
NM_016574
Gene ID (NCBI):
1813
Gene symbol
DRD2
Synonyms
D(2) dopamine receptor, D2DR, D2R, Dopamine D2 receptor, dopamine receptor D2, DRD2

Background

DRD2, also named as D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.


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