Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, SH-SY5Y cells, rat brain tissue |
| Positive IP detected in | SH-SY5Y cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 28 publications below |
| IHC | See 8 publications below |
| IF | See 13 publications below |
Product Information
55084-1-AP targets DRD2 in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | dopamine receptor D2 |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | NM_016574 |
| Gene Symbol | DRD2 |
| Gene ID (NCBI) | 1813 |
| RRID | AB_10859941 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14416 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DRD2, also named D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DRD2 antibody 55084-1-AP | Download protocol |
| IP protocol for DRD2 antibody 55084-1-AP | Download protocol |
| WB protocol for DRD2 antibody 55084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Non-canonical interplay between glutamatergic NMDA and dopamine receptors shapes synaptogenesis | ||
J Affect Disord Pramipexole improves depression-like behavior in diabetes mellitus with depression rats by inhibiting NLRP3 inflammasome-mediated neuroinflammation and preventing impaired neuroplasticity | ||
Int J Mol Sci Biochemical Neuroadaptations in the Rat Striatal Dopaminergic System after Prolonged Exposure to Methamphetamine Self-Administration | ||
J Neuroimmune Pharmacol 5-HT4 Receptor is Protective for MPTP-induced Parkinson's Disease Mice Via Altering Gastrointestinal Motility or Gut Microbiota |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH ARUNASALAM (Verified Customer) (05-29-2025) | This antibody worked for western blotting.
|
FH Ping (Verified Customer) (06-04-2019) | This antibody is good for both immunoprecipitation and western blot.
|
