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DRD2 Polyclonal antibody

DRD2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55084-1-AP

Synonyms

D(2) dopamine receptor, D2DR, D2R, Dopamine D2 receptor, dopamine receptor D2, DRD2



Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55084-1-AP targets DRD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name dopamine receptor D2
Calculated molecular weight 51 kDa
Observed molecular weight50-60 kDa
GenBank accession numberNM_016574
Gene symbol DRD2
Gene ID (NCBI) 1813
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DRD2, also named as D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.

Protocols

Product Specific Protocols
WB protocol for DRD2 antibody 55084-1-APDownload protocol
IHC protocol for DRD2 antibody 55084-1-APDownload protocol
IF protocol for DRD2 antibody 55084-1-APDownload protocol
IP protocol for DRD2 antibody 55084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Int Med Res

Mechanistic analysis of erectile dysfunction in a depression rat model.

Authors - Zhi-Ming Hong
ratIF

Andrologia

PMID: 30276840

Authors - Guotao Chen
ratWB

BMC Endocr Disord

The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.

Authors - Shuman Wang
ratWB

Clin Exp Pharmacol Physiol

Dopamine receptor D2 inhibition alleviates diabetic hepatic stellate cells fibrosis by regulating the TGF-β1/Smads and NFκB pathways.

Authors - Bingbing Zhao
ratIF

Andrology

Dopamine D2 receptors in the nucleus accumbens modulate erectile function in a rat model of nonorganic erectile dysfunction.

Authors - Guotao Chen
ratWB

J Ethnopharmacol

The ancient Chinese formula Longdan Xiegan Tang improves antipsychotic-induced hyperprolactinemia by repairing the hypothalamic and pituitary TGF-β1 signaling in rats.

Authors - Liying Ren

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ping (Verified Customer) (06-04-2019)

This antibody is good for both immunoprecipitation and western blot.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: rat brain tissue