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  • KD/KO Validated

DRD2 Antibody

DRD2 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55084-1-AP

Synonyms

D(2) dopamine receptor, D2DR, D2R, Dopamine D2 receptor, dopamine receptor D2, DRD2



Tested Applications

Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55084-1-AP targets DRD2 in IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name dopamine receptor D2
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa
GenBank accession number NM_016574
Gene symbol DRD2
Gene ID (NCBI) 1813
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DRD2, also named as D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.

Protocols

Product Specific Protocols
IHC protocol for DRD2 antibody 55084-1-APDownload protocol
IP protocol for DRD2 antibody 55084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Andrologia

Dopamine D2 receptors in the basolateral amygdala modulate erectile function in a rat model of nonorganic erectile dysfunction.

Authors - Guotao Chen
ratWB

J Ethnopharmacol

The ancient Chinese formula Longdan Xiegan Tang improves antipsychotic-induced hyperprolactinemia by repairing the hypothalamic and pituitary TGF-β1 signaling in rats.

Authors - Liying Ren
ratWB

J Ethnopharmacol

Paeoniflorin ameliorates antipsychotic-induced hyperprolactinemia in rats by attenuating impairment of the dopamine D2 receptor and TGF-β1 signaling pathways in the hypothalamus and pituitary.

Authors - Xiaoqian Huang
ratWB

Mol Brain

Development of a peptide targeting dopamine transporter to improve ADHD-like deficits.

Authors - Terence K Y Lai
humanWB

Mol Neurobiol

Protective Effect of Curcumin by Modulating BDNF/DARPP32/CREB in Arsenic-Induced Alterations in Dopaminergic Signaling in Rat Corpus Striatum.

Authors - Pranay Srivastava
humanWB

Cell

Dopamine Controls Systemic Inflammation through Inhibition of NLRP3 Inflammasome.

Authors - Yiqing Yan
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ping (Verified Customer) (06-04-2019)

This antibody is good for both immunoprecipitation and western blot.

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: rat brain tissue
No image submitted with review