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  • Western Blot (WB) analysis of HeLa cells using DUSP12 Polyclonal antibody (15667-1-AP)

    WB analysis of HeLa using 15667-1-AP

    WB result of DUSP12 antibody (15667-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DUSP12 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DUSP12 Polyclonal antibody (15667-1-AP)

    IHC staining of mouse brain using 15667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15667-1-AP (DUSP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using DUSP12 Polyclonal antibody (15667-1-AP)

    IHC staining of mouse kidney using 15667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15667-1-AP (DUSP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using DUSP12 Polyclonal antibody (15667-1-AP)

    IHC staining of mouse embryo using 15667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 15667-1-AP (DUSP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

DUSP12 Polyclonal antibody

DUSP12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15667-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

DUSP12, YVH1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue, mouse embryo tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15667-1-AP targets DUSP12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DUSP12 fusion protein Ag8131 Predict reactive species
Full Name dual specificity phosphatase 12
Calculated Molecular Weight 370 aa, 43 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC006286
Gene Symbol DUSP12
Gene ID (NCBI) 11266
RRIDAB_3085468
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUSP12 (Dual specificity protein phosphatase 12) belongs to the protein-tyrosine phosphatase family and non-receptor class dual specificity subfamily. DUSP12 is a kind of dual specificity phosphatase. It can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues and can dephosphorylate glucokinase in vitro (PMID:10446167). In addition, DUSP12 has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate and other in vitro substrates (PMID:24531476). DUSP12 is ubiquitously expressed in spleen, testis, ovary, and peripheral blood leukocytes and lower expression in liver and lung.

Protocols

Product Specific Protocols
WB protocol for DUSP12 antibody 15667-1-APDownload protocol
IHC protocol for DUSP12 antibody 15667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols