• Featured Product
  • KD/KO Validated

E2F1 Polyclonal antibody

E2F1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12171-1-AP

Synonyms

E2F 1, E2F transcription factor 1, E2F1, PBR3, PRB binding protein E2F 1, RBAP 1, RBAP1, RBBP 3, RBBP3, Transcription factor E2F1



Tested Applications

Positive WB detected inHeLa cells, A431 cells, mouse brain tissue, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

12171-1-AP targets E2F1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Artemia, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E2F1 fusion protein Ag2813
Full Name E2F transcription factor 1
Calculated molecular weight 437 aa, 47 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC050369
Gene symbol E2F1
Gene ID (NCBI) 1869
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of E2F1 protein, specifically recognizes the 47kd human E2F1 protein. The sumoylated E2F1 is bout 55 kDa.

Protocols

Product Specific Protocols
WB protocol for E2F1 antibody 12171-1-APDownload protocol
IHC protocol for E2F1 antibody 12171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Pharmacother

Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.

Authors - Yudi Xiong
humanWB

J Cell Mol Med

Down-regulation of TFAM increases the sensitivity of tumour cells to radiation via p53/TIGAR signalling pathway.

Authors - Xu Jiang
humanWB

Oncotarget

Long non-coding RNA HULC promotes tumor angiogenesis in liver cancer by up-regulating sphingosine kinase 1 (SPHK1).

Authors - Zhanping Lu
humanWB

Oncotarget

Dihydroartemisinin suppresses pancreatic cancer cells via a microRNA-mRNA regulatory network.

Authors - Yilong Li
humanWB

Oncotarget

RACK1 promotes lung cancer cell growth via an MCM7/RACK1/ Akt signaling complex.

Authors - Liangru Fei
humanWB

Tumour Biol

CIP2A is associated with multidrug resistance in cervical adenocarcinoma by a P-glycoprotein pathway.

Authors - Juan Liu