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EDA Polyclonal antibody

EDA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25892-1-AP

Synonyms

Ectodermal dysplasia protein, ectodysplasin A, ED1, ED1 A1, ED1 A2, EDA, EDA protein, EDA1, EDA2, HED, XHED, XLHED

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using EDA Polyclonal antibody (25892-1-AP)

    WB analysis of mouse liver using 25892-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25892-1-AP (EDA Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using EDA Polyclonal antibody (25892-1-AP)

    IHC staining of human liver using 25892-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25892-1-AP (EDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using EDA Polyclonal antibody (25892-1-AP)

    IHC staining of human liver using 25892-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25892-1-AP (EDA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25892-1-AP is EDA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EDA fusion protein Ag23154
Full Name ectodysplasin A
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC126143
Gene Symbol EDA
Gene ID (NCBI) 1896
RRIDAB_2880286
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EDA antibody 25892-1-APDownload protocol
IHC protocol for EDA antibody 25892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols