EFEMP2 Polyclonal antibody
EFEMP2 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12004-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse kidney tissue, K-562 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human heart tissue, human prostate cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
12004-1-AP targets EFEMP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EFEMP2 fusion protein Ag2640 |
| Full Name | EGF-containing fibulin-like extracellular matrix protein 2 |
| Calculated Molecular Weight | 443 aa, 49 kDa |
| Observed Molecular Weight | 52-55 kDa |
| GenBank Accession Number | BC010456 |
| Gene Symbol | EFEMP2 |
| Gene ID (NCBI) | 30008 |
| RRID | AB_10667002 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
| IHC protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
| IP protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Biol Ther STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2 | ||
Cell Mol Biol Lett EFEMP2 upregulates PD-L1 expression via EGFR/ERK1/2/c-Jun signaling to promote the invasion of ovarian cancer cells |
